Department of Chemical Engineering, Institute of Chemical Technology.
J Oleo Sci. 2021;70(4):559-570. doi: 10.5650/jos.ess20235.
The present study evaluates the potential use of ultrasound irradiation to synthesize decyl oleate using Fermase CALB10000 under the solvent-free system (SFS). The optimal condition to achieve a maximum yield of 97.14% was found to be 1:2 oleic acid:decanol ratio, 1.8% (w/w) enzyme loading, 45°C temperature, 200 rpm agitation speed, 50 W power input, 50% duty cycle, 22 kHz frequency and reaction time of 25 minutes. The thermodynamic study was done to determine the change in entropy, Gibb's free energy, and change in enthalpy at various temperatures. The experimental results and kinetic study showed that the reaction followed ordered bi-bi model with kinetic parameters as rate of reaction (V ) = 35.02 M/min/g catalyst, Michaelis constant for acid (K ) = 34.47 M, Michaelis constant for alcohol (K ) = 3.31 M, Inhibition constant (K) = 4542.4 M and sum of square error (SSE) = 0.000334. The application of ultrasound irradiation combined with biocatalyst and the absence of solvent intensified the process compared to the traditional stirring method using hexane as solvent.
本研究评估了在无溶剂体系（SFS）下使用 Fermase CALB10000 通过超声辐射合成癸酸辛酯的潜在用途。发现达到最大产率 97.14%的最佳条件为油酸：癸醇摩尔比 1:2，酶用量 1.8%（w/w），温度 45°C，搅拌速度 200rpm，功率输入 50W，占空比 50%，频率 22kHz，反应时间 25 分钟。进行了热力学研究以确定在不同温度下熵、吉布斯自由能和焓的变化。实验结果和动力学研究表明，该反应遵循有序双分子模型，动力学参数为反应速率（V）=35.02M/min/g 催化剂，酸的米氏常数（K）=34.47M，醇的米氏常数（K）=3.31M，抑制常数（K）=4542.4M，误差平方和（SSE）=0.000334。与使用正己烷作为溶剂的传统搅拌方法相比，超声辐射与生物催化剂的结合以及无溶剂的应用强化了该过程。
