Department of Microbiology and Immunology, School of Medicine, Soonchunhyang University, Cheonan, Republic of Korea.
Probiotics Microbiome Convergence Center, Asan, Republic of Korea.
Pol J Microbiol. 2021 Mar;70(1):117-130. doi: 10.33073/pjm-2021-011. Epub 2021 Mar 19.
is currently a significant multidrug-resistant bacterium, causing severe healthcare-associated and community-acquired infections worldwide. The current antibiotic regimen against this pathogen is becoming ineffective due to resistance, in addition, they disrupt the normal microbiota. It highlights the urgent need for a pathogen-specific drug with high antibacterial efficacy against . α-Viniferin, a bioactive phytochemical compound, has been reported to have excellent anti- efficacy as a topical agent. However, so far, there were no clinical trials that have been conducted to elucidate its efficacy. The present study aimed to investigate the antibacterial efficacy of α-viniferin against in a ten-day clinical trial. Based on the results, α-viniferin showed 50% minimum inhibitory concentrations (MIC values) of 7.8 μg/ml in culture broth medium. α-Viniferin was administered in the nares three times a day for ten days using a sterile cotton swab stick. Nasal swab specimens were collected before (0 days) and after finishing the trial (10 day), and then analyzed. In the culture and RT-PCR-based analysis, was reduced significantly: 0.01. In addition, 16S ribosomal RNA-based amplicon sequencing analysis showed that reduced from 51.03% to 23.99% at the genus level. RNA-seq analysis was also done to gain insights into molecular mechanisms of α-viniferin against , which revealed that some gene groups were reduced in 5-fold FC cutoff at two times MIC conditions. The study results demonstrate α-viniferin as a potential -specific drug candidate.
目前，是一种重要的多药耐药菌，在全球范围内导致严重的医疗保健相关和社区获得性感染。由于耐药性，目前针对这种病原体的抗生素方案已经失效，此外，它们还会破坏正常的微生物群。这凸显了迫切需要一种针对病原体的药物，该药物对具有高度抗菌功效。α-白皮杉醇是一种生物活性植物化学化合物，已被报道作为局部制剂具有出色的抗疗效。然而，到目前为止，还没有进行临床试验来阐明其疗效。本研究旨在调查α-白皮杉醇在为期十天的临床试验中对的抗菌功效。基于结果，α-白皮杉醇在培养肉汤培养基中的最小抑菌浓度（MIC 值）为 7.8μg/ml。使用无菌棉签棒，每天在鼻腔中给药三次，共十天。在试验前（0 天）和试验结束后（10 天）收集鼻拭子标本，然后进行分析。在培养和基于 RT-PCR 的分析中，显著减少：0.01。此外，16S 核糖体 RNA 扩增子测序分析显示，在属水平上从 51.03%减少到 23.99%。还进行了 RNA-seq 分析，以深入了解α-白皮杉醇对的分子机制，结果表明，在两次 MIC 条件下，一些基因组的减少了 5 倍 FC 截止值。研究结果表明，α-白皮杉醇是一种有潜力的针对病原体的药物候选物。
