Willemer S, Sames K, Lücht B
Medizinische Klinik, Universität Marburg.
Z Gerontol. 1988 Mar-Apr;21(2):74-8.
Cell nuclei of the livers of 12 male Wistar rats aged 24 months, and 16 male Wistar rats aged 4 months were isolated. 0.4 microCi [3H]-glucosamine/kg body weight were administered intraperitoneally. The livers of four animals were used for each biochemical analysis. The tissue was degraded by proteolysis, the glycosaminoglycans (GAGs) were isolated and the GAG types were determined by enzyme digestion, nitrite degradation and radiometry. The amount of GAGs was photometrically determined in single samples of pooled material of each of the two age groups. In paraffin sections (from other male Wistar rats of a different age), the influence of enzymes on specific GAG staining was observed. Most of the radioactivity was found in heparan sulfate (HS) and a lower content in chondroitin sulfates (CSs). In HS the incorporation increased with increasing age. The amount of HS showed no age-related differences. The results indicate an age-related activation of the HS metabolism. The GAG pattern and the age-related changes of the GAG types in total tissue (earlier results) are different from those in the nuclei. By histochemical methods, we observed a small but distinct effect of heparitinase in the cell nuclei.
分离出12只24月龄雄性Wistar大鼠和16只4月龄雄性Wistar大鼠肝脏的细胞核。腹腔注射0.4微居里[3H]-葡糖胺/千克体重。每次生化分析使用4只动物的肝脏。通过蛋白水解使组织降解，分离出糖胺聚糖（GAG），并通过酶消化、亚硝酸盐降解和放射测定法确定GAG类型。通过光度法测定两个年龄组各自合并材料的单个样品中GAG的量。在石蜡切片中（来自不同年龄的其他雄性Wistar大鼠），观察酶对特定GAG染色的影响。大部分放射性存在于硫酸乙酰肝素（HS）中，硫酸软骨素（CS）中的含量较低。在HS中，掺入量随年龄增长而增加。HS的量未显示出与年龄相关的差异。结果表明HS代谢存在与年龄相关的激活。总组织中GAG模式和GAG类型的年龄相关变化（早期结果）与细胞核中的不同。通过组织化学方法，我们观察到乙酰肝素酶在细胞核中有微小但明显的作用。
