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适用于向日葵霜霉病抗性标记辅助选择的SSR标记

SSR Markers Suitable for Marker Assisted Selection in Sunflower for Downy Mildew Resistance.

作者信息

Şahin Ezgi Çabuk, Kalenderoğlu Aral, Aydın Yıldız, Evci Göksel, Uncuoğlu Ahu Altınkut

机构信息

Department of Biology, Faculty of Science and Letters, Marmara University, Istanbul, 34722, Turkey.

Department of Bioengineering, Faculty of Engineering, Marmara University, Istanbul, 34722, Turkey.

出版信息

Open Life Sci. 2018 Sep 22;13:319-326. doi: 10.1515/biol-2018-0039. eCollection 2018 Jan.

Abstract

The effectiveness of genes is known to be resistant to downy mildew (DM) disease affected by fungus in sunflower. In this study phenotypic analysis was performed using inoculation tests and genotypic analysis were carried out with three DM resistance genes and . A total of 69 simple sequence repeat markers and 241 F individuals derived from a cross of RHA-419 (R) x P6LC (S), RHA-419 (R) x CL (S), RHA-419 (R) x OL (S), RHA419 (R) x 9758R (S), HA-R5 (R) x P6LC (S) and HA89 (R) x P6LC (S) parental lines were used to identify resistant hybrids in sunflower. Results of SSR analysis using markers linked with downy mildew resistance genes (, and ) and downy mildew inoculation tests were evaluated together and and (for ) markers showed positive correlation with their phenotypic results. These results suggest that these markers are associated with DM resistance and they can be used successfully in marker-assisted selection for sunflower breeding programs specific for downy mildew resistance.

摘要

已知向日葵中受真菌影响的霜霉病(DM)抗性基因的有效性。在本研究中,通过接种试验进行表型分析,并利用三个霜霉病抗性基因进行基因型分析。总共使用了69个简单序列重复标记和241个F个体,这些个体来自RHA - 419(R)×P6LC(S)、RHA - 419(R)×CL(S)、RHA - 419(R)×OL(S)、RHA419(R)×9758R(S)、HA - R5(R)×P6LC(S)和HA89(R)×P6LC(S)亲本系的杂交,以鉴定向日葵中的抗性杂种。将使用与霜霉病抗性基因(、和)连锁的标记进行的SSR分析结果和霜霉病接种试验结果一起评估,并且和(对于)标记与其表型结果呈正相关。这些结果表明,这些标记与霜霉病抗性相关,并且它们可以成功地用于针对霜霉病抗性的向日葵育种计划的标记辅助选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a018/7874726/bbc1e9281108/biol-13-319-g001.jpg

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