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五聚噻吩作为监测表没食子儿茶素没食子酸酯对成熟淀粉样纤维重塑活性的探针：克服硫黄素T的信号伪像

Pentameric Thiophene as a Probe to Monitor EGCG's Remodeling Activity of Mature Amyloid Fibrils: Overcoming Signal Artifacts of Thioflavin T.

作者信息

Kelley Mirian, Sant'Anna Ricardo, Fernandes Luiza, Palhano Fernando L

机构信息

Instituto de Bioquímica Médica Leopoldo de Meis, Programa de Biologia Estrutural, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil.

出版信息

ACS Omega. 2021 Mar 16;6(12):8700-8705. doi: 10.1021/acsomega.1c00680. eCollection 2021 Mar 30.

DOI:10.1021/acsomega.1c00680
PMID:33817533
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8015118/
Abstract

Thioflavin T fluorescence is a gold standard probe for the detection of amyloid fibrils. Herein, we showed that mature amyloid fibrils incubated with polyphenol epigallocatechin gallate (EGCG) present a fast reduction of the thioflavin T fluorescence, which is not related to remodeling activity. We propose the use of the pentameric thiophene fluorescence for monitoring the polyphenol remodeling activity.

摘要

硫黄素T荧光是检测淀粉样纤维的金标准探针。在此，我们表明，与多酚表没食子儿茶素没食子酸酯（EGCG）一起孵育的成熟淀粉样纤维会使硫黄素T荧光快速降低，这与重塑活性无关。我们建议使用五聚噻吩荧光来监测多酚重塑活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b78a/8015118/401815c83d78/ao1c00680_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b78a/8015118/28c606623063/ao1c00680_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b78a/8015118/15a29d81514f/ao1c00680_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b78a/8015118/38f4baf3d6fd/ao1c00680_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b78a/8015118/401815c83d78/ao1c00680_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b78a/8015118/28c606623063/ao1c00680_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b78a/8015118/15a29d81514f/ao1c00680_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b78a/8015118/38f4baf3d6fd/ao1c00680_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b78a/8015118/401815c83d78/ao1c00680_0005.jpg

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