Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP13630-970, Brazil.
Universidade de São Paulo, Faculdade de Medicina Veterinária e Ciência Animal, Departamento de Reprodução Animal, Pirassununga, Brazil.
Zygote. 2021 Oct;29(5):372-376. doi: 10.1017/S0967199421000125. Epub 2021 Apr 5.
Triploidization plays an important role in aquaculture and surrogate technologies. In this study, we induced triploidy in the matrinxã fish (Brycon amazonicus) using a heat-shock technique. Embryos at 2 min post fertilization (mpf) were heat shocked at 38°C, 40°C, or 42°C for 2 min. Untreated, intact embryos were used as a control. Survival rates during early development were monitored and ploidy status was confirmed using flow cytometry and nuclear diameter analysis of erythrocytes. The hatching rate reduced with heat-shock treatment, and heat-shock treatments at 42°C resulted in no hatching events. Optimal results were obtained at 40°C with 95% of larvae exhibiting triploidy. Therefore, we report that heat-shock treatments of embryos (2 mpf) at 40°C for 2 min is an effective way to induce triploid individuals in B. amazonicus.
三倍体化在水产养殖和代孕技术中起着重要作用。在这项研究中，我们使用热休克技术诱导马氏珠母贝（Brycon amazonicus）的三倍体。在受精后 2 分钟（mpf）时，将胚胎在 38°C、40°C 或 42°C 下热休克 2 分钟。未处理的完整胚胎用作对照。监测早期发育过程中的存活率，并使用流式细胞术和红细胞核直径分析确认倍性状态。热休克处理后孵化率降低，42°C 的热休克处理没有孵化事件。在 40°C 时获得最佳结果，95%的幼虫表现出三倍体。因此，我们报告在 40°C 下对 2 分钟的胚胎（2 mpf）进行热休克处理是诱导 B. amazonicus 三倍体个体的有效方法。
