Department of Pathology, Jilin Cancer Hospital, Changchun, China.
Department of Pathology, Changchun Obstetrics-Gynecology Hospital, Changchun, China.
Diagn Cytopathol. 2021 Jul;49(7):811-816. doi: 10.1002/dc.24745. Epub 2021 Apr 5.
The cytologic evaluation of serous effusions to distinguish malignant cells from reactive mesothelial cells (RMCs)was an enormous challenge. The purpose of this study was to investigate the diagnostic value of glucose transporter 1 (GLUT1) and calretinin (CR) in serous effusions of patients with malignant and in order to significantly ameliorate the diagnostic accuracy.
The expressions of GLUT1 and CR were measured by streptavidin-peroxidase (S-P) immunocytochemical technique in serous effusions of 183 patients with malignant and in 95 patients with benign diseases.
The positive ratio of GLUT1 was 91.8% (168/183) in serous effusions from patients with malignant and 5.3% (5/95) in benign diseases, they had a significant difference (P < .01). CR was expressed 89.5% (85/95) in benign diseases and 6.6% (12/183) in malignant, it also showed an important difference (P < 0.01). The combination of GLUT1 + CR revealed the best efficiency: the sensitivity and specificity were 100% and 98.9%, respectively.
Immunocytochemical staining for GLUT1 and CR may be used as a complementary tool for the detection of malignant effusions and help to make a distinction between cancer cells and RMCs. The combination of GLUT1 and CR with immunocytochemistry stained can be achieved a higher diagnostic performance.
鉴别浆膜腔积液中的恶性细胞与反应性间皮细胞（RMC）一直是一项艰巨的挑战。本研究旨在探讨葡萄糖转运蛋白 1（GLUT1）和钙视网膜蛋白（CR）在恶性浆膜腔积液中的诊断价值，以期显著提高诊断准确性。
采用链霉亲和素-过氧化物酶（S-P）免疫细胞化学技术检测 183 例恶性浆膜腔积液和 95 例良性疾病患者浆膜腔积液中 GLUT1 和 CR 的表达。
恶性浆膜腔积液中 GLUT1 的阳性率为 91.8%（168/183），良性疾病为 5.3%（5/95），差异有统计学意义（P<0.01）。CR 在良性疾病中的表达率为 89.5%（85/95），恶性疾病中为 6.6%（12/183），差异有统计学意义（P<0.01）。GLUT1+CR 联合检测效率最佳：敏感性和特异性分别为 100%和 98.9%。
GLUT1 和 CR 的免疫细胞化学染色可作为检测恶性积液的辅助工具，有助于区分癌细胞和 RMC。GLUT1 和 CR 联合免疫细胞化学染色可获得更高的诊断性能。
