Rodgers R, Crowl C P, Eimstad W M, Hu M W, Kam J K, Ronald R C, Rowley G L, Ullman E F
Clin Chem. 1978 Jan;24(1):95-100.
We describe a homogeneous enzyme immunoassay for measurement of cannabinoid metabolites, as well as delta9-tetrahydrocannabinol (I) in urine. Malate dehydrogenase from pig heart mitochondria was labeled with a derivative of I. The compound used to calibrate the assay was the I metabolite, 11-nor-delta9-tetrahydrocannabinol-9-carboxylic acid (II). With 15 microgram of II per liter of urine as the cutoff concentration, the assay can detect 25 microgram of II per liter with greater than 95% confidence. A positive response was obtained for urine specimens assayed within 30 min after exposure to cannabinoids. However, the persistence of metabolites of I in urine indicates that assay of this fluid is useful as an indicator of cannabinoid use but not as an indicator of intoxication.
我们描述了一种用于测量尿液中大麻素代谢物以及δ9-四氢大麻酚（I）的均相酶免疫测定法。来自猪心脏线粒体的苹果酸脱氢酶用I的衍生物进行标记。用于校准该测定法的化合物是I的代谢物11-去甲-δ9-四氢大麻酚-9-羧酸（II）。以每升尿液中15微克的II作为临界浓度，该测定法能够以大于95%的置信度检测出每升尿液中25微克的II。在接触大麻素后30分钟内检测的尿液标本获得了阳性反应。然而，I的代谢物在尿液中的持续存在表明，对这种液体进行检测可作为使用大麻素的指标，但不能作为中毒的指标。
