Drews U, Schleicher G, Stumpf W E, Thiedemann K U
Anatomisches Institut, Universität Tübingen, Federal Republic of Germany.
Anat Rec. 1988 Apr;220(4):393-6. doi: 10.1002/ar.1092200408.
Testicular feminization (Tfm) in the mouse is characterized by androgen insensitivity of the target cells. We describe the presence of androgen-insensitive Tfm cells in the epididymis of mosaic mice produced by converting female carriers of the Tfm mutation (XTfm/X+) to males via the sex reversal factor (Sxr). The mosaic arises by random X-inactivation. In the epididymal duct, flat undifferentiated Tfm cells are interspersed between high columnar wild-type cells. By thaw-mount autoradiography we show that after injection of [3H]dihydrotestosterone, radioactivity is concentrated in the nuclei of high columnar wild-type cells, whereas the nuclei of the low cuboid Tfm cells remain free of nuclear radioactivity. After injection of [3H]estradiol, both Tfm and wild-type cells show nuclear labeling. Our observations demonstrate that Tfm cells in the mosaic epididymis selectively lack nuclear dihydrotestosterone-binding sites, whereas estradiol-binding sites are intact.
小鼠睾丸女性化（Tfm）的特征是靶细胞对雄激素不敏感。我们描述了通过性反转因子（Sxr）将携带Tfm突变的雌性载体（XTfm/X+）转化为雄性所产生的嵌合小鼠附睾中存在雄激素不敏感的Tfm细胞。这种嵌合体是由随机的X染色体失活产生的。在附睾管中，扁平未分化的Tfm细胞散布在高柱状野生型细胞之间。通过冻片放射自显影术，我们发现注射[3H]双氢睾酮后，放射性集中在高柱状野生型细胞的细胞核中，而低立方状Tfm细胞的细胞核没有核放射性。注射[3H]雌二醇后，Tfm细胞和野生型细胞均显示核标记。我们的观察结果表明，嵌合附睾中的Tfm细胞选择性地缺乏核双氢睾酮结合位点，而雌二醇结合位点是完整的。
