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缺血缺氧预处理通过 miR-181a 信号增强移植嗅黏膜间充质干细胞在缺血性脑卒中后的线粒体功能恢复。

Ischemic-hypoxic preconditioning enhances the mitochondrial function recovery of transplanted olfactory mucosa mesenchymal stem cells via miR-181a signaling in ischemic stroke.

机构信息

The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410006, Hunan, P.R. China.

Hunan Provincical Key Laboratory of Neurorestoratology, The Second Affiliated Hospital Hunan Normal University, Changsha 410003, Hunan, P.R. China.

出版信息

Aging (Albany NY). 2021 Apr 4;13(8):11234-11256. doi: 10.18632/aging.202807.

DOI:10.18632/aging.202807
PMID:33820869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8109091/
Abstract

Cerebral ischemia/reperfusion injury causes a series of intricate cascade reactions in brain tissue causing apoptosis and proinflammatory programmed cell death known as pyroptosis of nerve cells. The dysfunction of target organelle mitochondria plays a key role in the process of neuronal apoptosis and pyroptosis. Mesenchymal stem cells (MSCs) have been widely used in the experimental or clinical treatment of various ischemic diseases, but the therapeutic efficacy of MSCs on cerebral ischemia-reperfusion injury need to be improved. We successfully cultured olfactory mucosa MSCs (OM-MSCs) to obtain a better source of seed cells. In this way, the therapeutic potential of OM-MSCs transplantation has been evaluated for ischemic stroke using an optimized culture scheme . Ischemic-hypoxic preconditioned OM-MSCs (IhOM-MSCs) were used to treat a neuron model of oxygen-glucose deprivation/reperfusion and the middle cerebral artery occlusion in rats. These results demonstrated that IhOM-MSCs mediated the upregulation of the downstream target genes GRP78 and Bcl-2 by miR-181a to protect mitochondrial function and inhibit apoptosis and pyroptosis of neurons in the ischemia/reperfusion injury model. Thus, IhOM-MSCs transplantation may be an effective therapy of ischemic stroke in the future.

摘要

脑缺血/再灌注损伤会导致脑组织内发生一系列复杂的级联反应,引起细胞凋亡和促炎程序性细胞死亡,即神经细胞的细胞焦亡。靶细胞器线粒体的功能障碍在神经元凋亡和细胞焦亡过程中起着关键作用。间充质干细胞(MSCs)已广泛应用于各种缺血性疾病的实验或临床治疗,但 MSCs 对脑缺血/再灌注损伤的治疗效果仍需提高。我们成功培养了嗅黏膜间充质干细胞(OM-MSCs),获得了更好的种子细胞来源。通过优化培养方案,评估了 OM-MSCs 移植治疗缺血性脑卒中的治疗潜力。采用缺血缺氧预处理的 OM-MSCs(IhOM-MSCs)治疗氧葡萄糖剥夺/再灌注诱导的神经元模型和大鼠大脑中动脉闭塞模型。这些结果表明,IhOM-MSCs 通过 miR-181a 介导下游靶基因 GRP78 和 Bcl-2 的上调,保护线粒体功能,抑制缺血再灌注损伤模型中神经元的凋亡和细胞焦亡。因此,IhOM-MSCs 移植可能是未来缺血性脑卒中的一种有效治疗方法。

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本文引用的文献

1
[Development of DDS Actively to Overcome the Blood-brain Barrier in the Region of Ischemic Stroke].积极开发药物递送系统以克服缺血性中风区域的血脑屏障
Yakugaku Zasshi. 2020;140(8):1007-1012. doi: 10.1248/yakushi.20-00012-7.
2
Involvement of ROS/NLRP3 Inflammasome Signaling Pathway in Doxorubicin-Induced Cardiotoxicity.活性氧(ROS)/NLRP3 炎性小体信号通路在多柔比星致心肌毒性中的作用。
Cardiovasc Toxicol. 2020 Oct;20(5):507-519. doi: 10.1007/s12012-020-09576-4.
3
Hypoxia-preconditioned olfactory mucosa mesenchymal stem cells abolish cerebral ischemia/reperfusion-induced pyroptosis and apoptotic death of microglial cells by activating HIF-1α.
明胶海绵支架递送的嗅黏膜间充质干细胞促进脊髓损伤的功能恢复。
Front Bioeng Biotechnol. 2025 Jul 9;13:1628758. doi: 10.3389/fbioe.2025.1628758. eCollection 2025.
4
Role of miR-181 Family Members in Stroke: Insights into Mechanisms and Therapeutic Potential.miR-181家族成员在中风中的作用:对机制及治疗潜力的见解
Int J Mol Sci. 2025 Jan 7;26(2):440. doi: 10.3390/ijms26020440.
5
Transplantation of neural stem cells improves recovery of stroke-affected mice and induces cell-specific changes in GSDMD and MLKL expression.神经干细胞移植可改善中风小鼠的恢复情况,并诱导GSDMD和MLKL表达发生细胞特异性变化。
Front Mol Neurosci. 2024 Aug 15;17:1439994. doi: 10.3389/fnmol.2024.1439994. eCollection 2024.
6
TGF-β1 mediates hypoxia-preconditioned olfactory mucosa mesenchymal stem cells improved neural functional recovery in Parkinson's disease models and patients.TGF-β1 介导低氧预处理嗅黏膜间充质干细胞改善帕金森病模型及患者的神经功能恢复。
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7
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8
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4
Blood-brain barrier integrity of stroke patients presenting in an extended time window.发病时间窗延长的脑卒中患者的血脑屏障完整性。
BMC Neurol. 2020 Feb 13;20(1):54. doi: 10.1186/s12883-020-01634-2.
5
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Biomed Pharmacother. 2020 Apr;124:109834. doi: 10.1016/j.biopha.2020.109834. Epub 2020 Jan 21.
6
Early Transplantation of Human Cranial Bone-derived Mesenchymal Stem Cells Enhances Functional Recovery in Ischemic Stroke Model Rats.人颅骨源性间充质干细胞早期移植增强缺血性脑卒中模型大鼠的功能恢复。
Neurol Med Chir (Tokyo). 2020 Feb 15;60(2):83-93. doi: 10.2176/nmc.oa.2019-0186. Epub 2020 Jan 18.
7
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