Unité Biologie des ARN des Pathogènes Fongiques, Département de Mycologie, Institut Pasteur, F-75015 Paris, France.
Institut Pasteur, HUB Bioinformatique et Biostatistique, C3BI, USR 3756 IP CNRS, F-75015 Paris, France.
G3 (Bethesda). 2021 Jun 17;11(6). doi: 10.1093/g3journal/jkab107.
The genomes of a large number of Cryptococcus neoformans isolates have been sequenced and analyzed in recent years. These genomes have been used to understand the global population structure of this opportunistic pathogen. However, only a small number of South American isolates have been considered in these studies, and the population structure of C. neoformans in this part of the world remains elusive. Here, we analyzed the genomic sequences of 53 Brazilian Cryptococcus isolates and deciphered the C. neoformans population structure in this country. Our data reveal an African-like structure that suggested repeated intercontinental transports from Africa to South America. We also identified a mutator phenotype in one VNBII Brazilian isolate, exemplifying how fast-evolving isolates can shape the Cryptococcus population structure. Finally, phenotypic analyses revealed wide diversity but not lineage specificity in the expression of classical virulence traits within the set of isolates.
近年来，大量新型隐球菌分离株的基因组已被测序和分析。这些基因组被用于了解这种机会性病原体的全球种群结构。然而，在这些研究中只考虑了少数南美分离株，而该地区新型隐球菌的种群结构仍然难以捉摸。在这里，我们分析了 53 株巴西新型隐球菌分离株的基因组序列，并破译了该国新型隐球菌的种群结构。我们的数据显示出一种类似非洲的结构，表明该结构是由非洲反复向南美洲的跨洲际传播造成的。我们还在一个 VNBII 巴西分离株中发现了一个突变体表型，说明了快速进化的分离株如何塑造新型隐球菌的种群结构。最后，表型分析显示，在一组分离株中，经典毒力表型的表达存在广泛的多样性，但没有谱系特异性。
