美国内华达州利用酶联免疫吸附试验检测美洲黑熊（Ursus americanus）弓形虫血清抗体阳性率。

Seroprevalence of Toxoplasma gondii in American Black Bears (Ursus americanus) in Nevada, USA, using an Enzyme-linked Immunosorbent Assay.

机构信息

Carlson College of Veterinary Medicine, Oregon State University, Magruder Hall, 700 SW 30th St., Corvallis, Oregon 97331, USA.

Oregon Veterinary Diagnostic Laboratory, Carlson College of Veterinary Medicine, Magruder Hall, 700 SW 30th St., Oregon State University, Corvallis, Oregon 97331, USA.

出版信息

J Wildl Dis. 2021 Apr 1;57(2):408-412. doi: 10.7589/JWD-D-20-00066.

DOI:10.7589/JWD-D-20-00066

PMID:33822154

Abstract

Archived serum samples taken between 1997 and 2017 from 170 American black bears (Ursus americanus) in the Lake Tahoe area between California and Nevada, US, were tested for Toxoplasma antibodies to assess the seroprevalence of this agent. Samples were screened using a commercial porcine Toxoplasma (enzyme-linked immunosorbent assay [ELISA]) modified with Protein A/G peroxidase and compared to a traditional fluorescent antibody test. Results were analyzed to determine if there were differences in seroprevalence based on the test used, sex of bears, or habitat usage (urban-suburban vs. wildland). No significant differences in seroprevalence were attributable to any of these parameters. The overall seropositivity for bears was 36% (62/170), with urban-suburban bears scoring lower (31%; 37/119) than rural-wildland bears (40%; 18/45). Our results strongly support the use of a Protein A/G-modified ELISA for determining Toxoplasma exposure in black bears. We found somewhat lower levels of Toxoplasma antibodies in black bears from this region than in several reports from populations in the eastern US.

摘要

从 1997 年至 2017 年，在美国加利福尼亚州和内华达州之间的太浩湖地区，对 170 头美洲黑熊（Ursus americanus）的存档血清样本进行了检测，以评估该病原体的血清阳性率。使用商业猪源弓形虫（酶联免疫吸附试验[ELISA]）进行了检测，该方法经过改良，使用了蛋白 A/G 过氧化物酶，并与传统的荧光抗体检测进行了比较。分析结果以确定是否基于使用的检测方法、熊的性别或栖息地使用（城市-郊区与野外）存在血清阳性率差异。这些参数都没有导致血清阳性率出现显著差异。熊的总血清阳性率为 36%（62/170），城市-郊区熊的血清阳性率（31%；37/119）低于农村-野外熊的血清阳性率（40%；18/45）。我们的结果强烈支持使用改良的蛋白 A/G-ELISA 来确定黑熊的弓形虫暴露情况。我们发现该地区的黑熊的弓形虫抗体水平略低于美国东部几个种群的报道。