Collaborative Innovation Center of Zhejiang Green Pesticide, National Joint Local Engineering Laboratory of Biopesticide High-Efficient Preparation, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, People's Republic of China.
Phytopathology. 2021 Nov;111(11):1918-1926. doi: 10.1094/PHYTO-11-20-0490-R. Epub 2021 Nov 7.
species complex (RSSC) contains a group of destructive plant pathogenic bacteria, causing bacterial wilt of >200 species of crops and trees, such as , worldwide. RSSC can survive in the soil environment for a long time and start infection after activation by host plants. This study conducted a transcriptome analysis on the expression pattern of the pathogenicity-related genes of a new isolated RSSC strain YQ ( phylotype I-16) in response to cladophyll (a branch of a stem that resembles and functions as a leaf) and root debris under in vitro culture. The cladophyll debris induced more genes up-regulated than the root debris, including pathogenicity-related genes involved in motility, effectors, type III secretion systems, quorum sensing, and plant cell wall degradation. Besides, many differentially expressed genes were related to transcriptional regulator such as cyclic dimeric guanosine monophosphate. Moreover, the cultures with cladophyll debris induced a faster wilting in bioassays, and the cell swimming was enhanced by cladophyll exudate. cladophylls could activate the expression of pathogenicity-related genes of strain YQ and accelerate infection. Our findings suggest that litterfall management in forests, or even other plantations, should receive attention to prevent the induction of bacterial wilt disease caused by RSSC.
物种复合体（RSSC）包含一组具有破坏性的植物病原细菌，能引起 200 多种作物和树木的细菌性萎蔫病，在全球范围内造成严重影响。RSSC 可以在土壤环境中长时间存活，并在被宿主植物激活后开始感染。本研究对一株新分离的 RSSC 菌株 YQ（I-16 型）在体外培养中对 叶状体和根碎片的反应进行了转录组分析，以研究其与致病性相关的基因表达模式。叶状体碎片诱导的上调基因多于根碎片，包括与运动性、效应子、III 型分泌系统、群体感应和植物细胞壁降解相关的致病性相关基因。此外，许多差异表达基因与环二鸟苷酸等转录调节因子有关。此外，含有叶状体碎片的培养物在生物测定中引起更快的萎蔫，并且叶状体渗出物增强了细胞游动性。叶状体可以激活菌株 YQ 与致病性相关的基因表达，并加速感染。我们的研究结果表明，森林中的凋落物管理，甚至其他种植园的凋落物管理，都应该得到重视，以防止 RSSC 引起的细菌性萎蔫病的发生。
