Funae Y, Imaoka S, Shimojo N
Osaka City University Medical School, Japan.
Biochem Int. 1988 Mar;16(3):503-9.
A diabetes-inducible form of cytochrome P-450, termed P-450DM, was purified to electrophoretical homogeneity (MW 51,000) by high-performance liquid chromatography from liver microsomes of diabetic rats induced with streptozotocin. The CO-reduced absorption maximum of P-450DM was at 452 nm and the oxidized heme iron appeared to be predominately in the high-spin state as deduced from the Soret maximum at 395 nm. P-450DM was active in aniline hydroxylation and N-nitrosodimethylamine demethylation. The dealkylation activity toward 7-ethoxycoumarin by P-450DM was much enhanced by the addition of cytochrome b5.
一种可被糖尿病诱导产生的细胞色素P-450，称为P-450DM，通过高效液相色谱法从用链脲佐菌素诱导的糖尿病大鼠的肝微粒体中纯化至电泳纯（分子量51,000）。P-450DM的一氧化碳还原吸收峰在452nm处，从395nm处的Soret峰推断，氧化血红素铁似乎主要处于高自旋状态。P-450DM在苯胺羟基化和N-亚硝基二甲胺脱甲基反应中具有活性。添加细胞色素b5可大大增强P-450DM对7-乙氧基香豆素的脱烷基活性。
