• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

杏贝滴鼻剂对变应性鼻炎豚鼠鼻黏膜成纤维细胞Fyn-STAT5通路的影响

Effect of Xingbi Gel Nasal Drops on Fyn-STAT5 Pathway in Nasal Mucosa Fibroblasts of Guinea Pigs with Allergic Rhinitis.

作者信息

Zhuang Xiangli, Wu Bo, Qiu Caixia, Ai Si, Zheng Jian

机构信息

Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.

Second People's Hospital, Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China.

出版信息

Evid Based Complement Alternat Med. 2021 Mar 20;2021:6686815. doi: 10.1155/2021/6686815. eCollection 2021.

DOI:10.1155/2021/6686815
PMID:33824677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8007362/
Abstract

Fyn-STAT5 is considered to be the frontier signaling pathway of IgE-mediated allergic reactions related to mast cell activation, but research on allergic rhinitis (AR) has been rarely reported. Xingbi gel nasal drops (XGND) are a compound preparation of traditional Chinese medicine, which has the exact therapeutic efficacy on AR. The current study aimed to observe the effects of XGND on Fyn-STAT5 pathway in AR guinea pig nasal mucosal fibroblasts and further illuminate the possible therapeutic mechanism of XGND on AR. The isolated and cultured nasal mucosa fibroblasts from AR guinea pigs were identified by immunocytochemical staining. Real-time PCR and western blot were performed to detect the mRNA and protein levels of the Fyn-STAT5 pathway and related cytokines in AR guinea pig nasal mucosal fibroblasts. The results indicated that XGND may interfere with the Fyn-STAT5 pathway by reducing the expression of Fyn and SCF and upregulating STAT5 and IL-10, thereby inhibiting proliferation and degranulation of mast cells, correcting Th1/Th2 immune imbalance, and then alleviating the immune response of AR fibroblasts. Our study revealed the possible regulatory mechanism of XGND in AR and laid an experimental foundation for improving the clinical efficacy of AR and enriching the clinical medication for AR.

摘要

Fyn-STAT5被认为是与肥大细胞活化相关的IgE介导的过敏反应的前沿信号通路，但关于变应性鼻炎（AR）的研究报道很少。辛荜滴鼻凝胶（XGND）是一种中药复方制剂，对AR具有确切的治疗效果。本研究旨在观察XGND对AR豚鼠鼻黏膜成纤维细胞中Fyn-STAT5通路的影响，并进一步阐明XGND对AR的可能治疗机制。采用免疫细胞化学染色法对从AR豚鼠中分离培养的鼻黏膜成纤维细胞进行鉴定。运用实时荧光定量PCR和蛋白质印迹法检测AR豚鼠鼻黏膜成纤维细胞中Fyn-STAT5通路及相关细胞因子的mRNA和蛋白水平。结果表明，XGND可能通过降低Fyn和SCF的表达，上调STAT5和IL-10来干扰Fyn-STAT5通路，从而抑制肥大细胞的增殖和脱颗粒，纠正Th1/Th2免疫失衡，进而减轻AR成纤维细胞的免疫反应。我们的研究揭示了XGND在AR中的可能调控机制，为提高AR的临床疗效和丰富AR的临床用药奠定了实验基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/12f432a5677e/ECAM2021-6686815.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/f0c5f0cc0924/ECAM2021-6686815.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/ad011d935dbd/ECAM2021-6686815.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/9048357e9223/ECAM2021-6686815.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/e10edc6e07af/ECAM2021-6686815.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/12f432a5677e/ECAM2021-6686815.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/f0c5f0cc0924/ECAM2021-6686815.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/ad011d935dbd/ECAM2021-6686815.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/9048357e9223/ECAM2021-6686815.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/e10edc6e07af/ECAM2021-6686815.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c24/8007362/12f432a5677e/ECAM2021-6686815.005.jpg

相似文献

1
Effect of Xingbi Gel Nasal Drops on Fyn-STAT5 Pathway in Nasal Mucosa Fibroblasts of Guinea Pigs with Allergic Rhinitis.杏贝滴鼻剂对变应性鼻炎豚鼠鼻黏膜成纤维细胞Fyn-STAT5通路的影响
Evid Based Complement Alternat Med. 2021 Mar 20;2021:6686815. doi: 10.1155/2021/6686815. eCollection 2021.
2
Effects of Xingbi gel on leukotriene E4 and immunoglobulin E production and nasal eosinophilia in a guinea pig model for allergic rhinitis.兴比凝胶对变应性鼻炎豚鼠模型白三烯 E4 和免疫球蛋白 E 产生及鼻嗜酸性粒细胞的影响。
Asian Pac J Allergy Immunol. 2015 Jun;33(2):99-106. doi: 10.12932/AP0473.33.1.201510.12932/AP0520.33.2.2015.
3
Xinqin exhibits the anti-allergic effect through the JAK2/STAT5 signaling pathway.辛芩通过JAK2/STAT5信号通路发挥抗过敏作用。
J Ethnopharmacol. 2016 Dec 4;193:466-473. doi: 10.1016/j.jep.2016.09.045. Epub 2016 Sep 23.
4
Inhibitory effect of baicalin on allergic response in ovalbumin-induced allergic rhinitis guinea pigs and lipopolysaccharide-stimulated human mast cells.黄芩苷对卵清蛋白诱导的变应性鼻炎豚鼠和脂多糖刺激的人肥大细胞过敏反应的抑制作用。
Inflamm Res. 2016 Aug;65(8):603-12. doi: 10.1007/s00011-016-0943-0. Epub 2016 Apr 4.
5
Xingbi Gel Ameliorates Allergic Rhinitis by Regulating IFN-γ Gene Promoter Methylation in CD4+ T Cells the ERK-DNMT Pathway.杏贝凝胶通过ERK-DNMT途径调节CD4+ T细胞中IFN-γ基因启动子甲基化来改善变应性鼻炎
Front Surg. 2021 Feb 15;7:619053. doi: 10.3389/fsurg.2020.619053. eCollection 2020.
6
Hydrogen-Rich Saline Ameliorates Allergic Rhinitis by Reversing the Imbalance of Th1/Th2 and Up-Regulation of CD4+CD25+Foxp3+Regulatory T Cells, Interleukin-10, and Membrane-Bound Transforming Growth Factor-β in Guinea Pigs.富氢生理盐水通过逆转 Th1/Th2 失衡和上调 CD4+CD25+Foxp3+调节性 T 细胞、白细胞介素-10 和膜结合转化生长因子-β来改善豚鼠变应性鼻炎。
Inflammation. 2018 Feb;41(1):81-92. doi: 10.1007/s10753-017-0666-6.
7
The Anti-Allergic Rhinitis Effect of Traditional Chinese Medicine of Shenqi by Regulating Mast Cell Degranulation and Th1/Th2 Cytokine Balance.参芪中药通过调节肥大细胞脱颗粒和Th1/Th2细胞因子平衡发挥抗变应性鼻炎的作用。
Molecules. 2017 Mar 22;22(3):504. doi: 10.3390/molecules22030504.
8
Desmoglein 3 gene mediates epidermal growth factor/epidermal growth factor receptor signaling pathway involved in inflammatory response and immune function of anaphylactic rhinitis.桥粒芯糖蛋白 3 基因介导表皮生长因子/表皮生长因子受体信号通路参与变应性鼻炎的炎症反应和免疫功能。
Biomed Pharmacother. 2019 Oct;118:109214. doi: 10.1016/j.biopha.2019.109214. Epub 2019 Aug 2.
9
Changes in IL-4 and IL-13 expression in allergic-rhinitis treated with hydrogen-rich saline in guinea-pig model.富氢盐水治疗豚鼠变应性鼻炎模型中白细胞介素-4和白细胞介素-13表达的变化
Allergol Immunopathol (Madr). 2017 Jul-Aug;45(4):350-355. doi: 10.1016/j.aller.2016.10.007. Epub 2017 Feb 16.
10
H2-Eb1 expression is upregulated in the nasal mucosa of allergic rhinitis.H2-Eb1在变应性鼻炎鼻黏膜中表达上调。
Asian Pac J Allergy Immunol. 2014 Dec;32(4):308-15. doi: 10.12932/AP0478.32.4.2014.

本文引用的文献

1
Inhibitory effects of bisdemethoxycurcumin on mast cell-mediated allergic diseases.双去甲氧基姜黄素对肥大细胞介导的过敏性疾病的抑制作用。
Int Immunopharmacol. 2018 Dec;65:182-189. doi: 10.1016/j.intimp.2018.10.005. Epub 2018 Oct 10.
2
FYN promotes mesenchymal phenotypes of basal type breast cancer cells through STAT5/NOTCH2 signaling node.FYN 通过 STAT5/NOTCH2 信号节点促进基底型乳腺癌细胞的间质表型。
Oncogene. 2018 Apr;37(14):1857-1868. doi: 10.1038/s41388-017-0114-y. Epub 2018 Jan 19.
3
Simultaneous induction of HSP70 expression, and degranulation, in IgE/Ag-stimulated or extracellular HSP70-stimulated mast cells.
同时诱导 IgE/Ag 刺激或细胞外 HSP70 刺激的肥大细胞中 HSP70 的表达和脱颗粒。
Allergy. 2018 Feb;73(2):361-368. doi: 10.1111/all.13296. Epub 2017 Oct 17.
4
Small-molecule studies identify CDK8 as a regulator of IL-10 in myeloid cells.小分子研究确定CDK8是髓系细胞中白细胞介素-10的调节因子。
Nat Chem Biol. 2017 Oct;13(10):1102-1108. doi: 10.1038/nchembio.2458. Epub 2017 Aug 14.
5
Diesel Exhaust Particles Upregulate Interleukins IL-6 and IL-8 in Nasal Fibroblasts.柴油尾气颗粒上调鼻成纤维细胞中的白细胞介素IL-6和IL-8 。
PLoS One. 2016 Jun 13;11(6):e0157058. doi: 10.1371/journal.pone.0157058. eCollection 2016.
6
FYN expression potentiates FLT3-ITD induced STAT5 signaling in acute myeloid leukemia.FYN表达增强急性髓系白血病中FLT3-ITD诱导的STAT5信号传导。
Oncotarget. 2016 Mar 1;7(9):9964-74. doi: 10.18632/oncotarget.7128.
7
The immune-modulatory effects of a mixed herbal formula on dendritic cells and CD4+ T lymphocytes in the treatment of dust mite allergy asthma and perennial allergic rhinitis.一种混合草药配方对树突状细胞和CD4+ T淋巴细胞在治疗尘螨过敏性哮喘和常年性变应性鼻炎中的免疫调节作用。
J Asthma. 2016;53(4):446-51. doi: 10.3109/02770903.2015.1104692. Epub 2016 Jan 21.
8
The Transcription Factor Ehf Is Involved in TGF-β-Induced Suppression of FcεRI and c-Kit Expression and FcεRI-Mediated Activation in Mast Cells.转录因子Ehf参与转化生长因子-β诱导的肥大细胞中FcεRI和c-Kit表达的抑制以及FcεRI介导的激活。
J Immunol. 2015 Oct 1;195(7):3427-35. doi: 10.4049/jimmunol.1402856. Epub 2015 Aug 21.
9
Effects of Xingbi gel on leukotriene E4 and immunoglobulin E production and nasal eosinophilia in a guinea pig model for allergic rhinitis.兴比凝胶对变应性鼻炎豚鼠模型白三烯 E4 和免疫球蛋白 E 产生及鼻嗜酸性粒细胞的影响。
Asian Pac J Allergy Immunol. 2015 Jun;33(2):99-106. doi: 10.12932/AP0473.33.1.201510.12932/AP0520.33.2.2015.
10
Clinical practice guideline: allergic rhinitis executive summary.临床实践指南：变应性鼻炎执行摘要
Otolaryngol Head Neck Surg. 2015 Feb;152(2):197-206. doi: 10.1177/0194599814562166.