Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People's Republic of China.
College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, People's Republic of China.
Mikrochim Acta. 2021 Apr 6;188(5):156. doi: 10.1007/s00604-021-04802-4.
A MnO nanosheet-assisted ratiometric fluorescence probe based on carbon quantum dots (CQDs) and o-phenylenediamine (OPD) has been developed for the detection of the anticancer drug 6-mercaptopurine (6-MP). CQDs with strong fluorescence are synthesized via the one-step hydrothermal method. MnO nanosheets as an oxidase-mimicking nanomaterial directly oxidize OPD into 2,3-diaminophenazine (DAP) which has a fluorescence emission at 570 nm, whereas the fluorescence of CQDs at 445 nm is then reduced by the DAP through the inner filter effect (IFE) under a single excitation wavelength (370 nm). After adding 6-MP, MnO nanosheets can be reduced to Mn and lose their oxidase-like property, blocking the IFE with the fluorescence decrease of DAP and fluorescence increase of CQDs. The novel ratiometric fluorescence probe exhibits considerable sensitivity toward 6-MP and linear response is in the 0.46-100.0 μmol L concentration range with the detection limit of 0.14 μmol L. Furthermore, the probe shows good selectivity when exposed to a series of interfering other organic and inorganic compounds, and biomolecules and can be applied to the detection for 6-MP in human serum samples and pharmaceutical tablets. Satisfactory recoveries of 6-MP in human serum samples are in the range 96.1-110.9% with the RSD of 1.4 to 3.2%. The amount of 6-MP is successfully estimated as 49.3 mg in pharmaceutical tablet with the RSD of about 2.2%.
一种基于碳量子点（CQDs）和邻苯二胺（OPD）的 MnO 纳米片辅助比率荧光探针已被开发用于检测抗癌药物 6-巯基嘌呤（6-MP）。通过一步水热法合成具有强荧光的 CQDs。MnO 纳米片作为一种氧化酶模拟纳米材料，可直接将 OPD 氧化成具有 570nm 荧光发射的 2,3-二氨基吩嗪（DAP），而在单一激发波长（370nm）下，DAP 通过内滤效应（IFE）可使 445nm 的 CQDs 荧光减少。加入 6-MP 后，MnO 纳米片可还原为 Mn 并失去其氧化酶样性质，从而阻断 IFE，使 DAP 的荧光减少和 CQDs 的荧光增加。新型比率荧光探针对 6-MP 具有相当高的灵敏度，线性响应范围为 0.46-100.0μmol L，检测限为 0.14μmol L。此外，该探针在暴露于一系列干扰有机和无机化合物以及生物分子时表现出良好的选择性，可用于人血清样品和药片药物中 6-MP 的检测。人血清样品中 6-MP 的回收率在 96.1-110.9%范围内，RSD 为 1.4-3.2%。成功地估计了药片药物中 6-MP 的含量为 49.3mg，RSD 约为 2.2%。
