灌注大鼠肾脏中支链酰基肉碱和支链羟酸的形成与排泄

Formation and excretion of branched-chain acylcarnitines and branched-chain hydroxy acids in the perfused rat kidney.

作者信息

Hokland B M, Bremer J

机构信息

Institute of Medical Biochemistry, University of Oslo, Norway.

出版信息

Biochim Biophys Acta. 1988 Jul 1;961(1):30-7. doi: 10.1016/0005-2760(88)90127-0.

DOI:10.1016/0005-2760(88)90127-0

PMID:3382691

Abstract

alpha-Keto[U-14C]isovalerate, alpha-keto[U-14C]isocaproate and alpha-keto[U-14C]beta-methylvalerate are metabolized in the perfused kidney. Labelled 3-hydroxyisobutyrate, 3-hydroxyisovalerate, 2-methyl-3-hydroxybutyrate, branched-chain amino acids, branched-chain acylcarnitines and lactate are formed. Hydroxy acids and acylcarnitines are excreted preferentially in the urine, whereas the branched-chain amino acids are preferentially excreted in the perfusate. There is no accumulation of (U-14C)-labelled alpha-keto acids or labelled metabolites in the kidney during perfusion. (-)-Carnitine accumulates rapidly in the kidney when it is added to the perfusate. A high kidney carnitine level enhances the excretion of carnitine esters in the urine.

摘要

α-酮[U-¹⁴C]异戊酸、α-酮[U-¹⁴C]异己酸和α-酮[U-¹⁴C]β-甲基戊酸在灌注肾中代谢。形成了标记的3-羟基异丁酸、3-羟基异戊酸、2-甲基-3-羟基丁酸、支链氨基酸、支链酰基肉碱和乳酸。羟基酸和酰基肉碱优先经尿液排泄，而支链氨基酸优先经灌注液排泄。灌注过程中，肾内不会积累（U-¹⁴C）标记的α-酮酸或标记代谢物。当（-）-肉碱添加到灌注液中时，它会在肾内迅速积累。高肾肉碱水平会增强肉碱酯经尿液的排泄。