DiCesare Nicolas, Lakowicz Joseph R
Center for Fluorescence Spectroscopy, University of Maryland, School of Medicine, 725 W. Lombard St., Baltimore, MD 21201, USA.
Tetrahedron Lett. 2002 Apr 1;43(14):2615-2618. doi: 10.1016/s0040-4039(02)00312-x. Epub 2002 Mar 6.
Two new fluorescent probes based on 1,3-diphenylprop-2-en-1-one and on 1,5-diphenylpenta-2,4-dien-1-one structures are presented. Both probes posses one electron-donating dimethylamino group and one boronic acid group (electron-withdrawing group). The change between the neutral and the anionic form of the boronic acid group induced at high pH and/or in presence of sugar, induces optical changes for both probes. Spectroscopic data, p and dissociation constants for different monosaccharides are presented and discussed in terms of sugar detection.
本文介绍了两种基于1,3 - 二苯基丙 - 2 - 烯 - 1 - 酮和1,5 - 二苯基戊 - 2,4 - 二烯 - 1 - 酮结构的新型荧光探针。两种探针均具有一个供电子的二甲氨基基团和一个硼酸基团（吸电子基团）。在高pH值和/或存在糖的情况下，硼酸基团的中性和阴离子形式之间的变化会引起两种探针的光学变化。文中给出了光谱数据、不同单糖的pKa和离解常数，并就糖检测方面进行了讨论。
