Zhang Yi, Zhang Hai Ying, Yan Jin Jin, Xia Meng Ning, Liu Ying Long, Lu Yan Mei, Yan Yun Zhi, Cheng Shuang Huai
Public Research Lab of Hainan Medical University, Haikou, China.
Anhui Health College, Guichi, China.
Mitochondrial DNA B Resour. 2021 Mar 26;6(3):1194-1196. doi: 10.1080/23802359.2021.1903351.
The complete mitochondrial genome of was sequenced using primers design, clone culture, DNA extraction, LONG-PCR amplification, purification and clone sequencing. We found that it is composed of two circular chromosomes, designated mtDNA I (11,398 bp) and mtDNA II (12,820 bp). The gene content of the mitochondrial genome was similar to that of the previously reported mitochondrial genome of . It contained 22 tRNA genes, 2 rRNA genes and 12 protein-coding genes (PCGs). Four of the 12 PCGs had an incomplete stop codons, TA（cob, atp6, nd3）or T(cox3). The A + T content of mitochondrial genome was apparently higher (mtDNA-I 70.2% and mtDNA II 70.4%) than that of the mitochondrial genome of (mtDNA-I 63.9% and mtDNA-II 62.9%).
使用引物设计、克隆培养、DNA提取、长链PCR扩增、纯化和克隆测序对[物种名称]的完整线粒体基因组进行了测序。我们发现它由两个环状染色体组成，分别命名为线粒体DNA I（11,398 bp）和线粒体DNA II（12,820 bp）。[物种名称]线粒体基因组的基因内容与先前报道的[另一物种名称]线粒体基因组相似。它包含22个tRNA基因、2个rRNA基因和12个蛋白质编码基因（PCGs）。12个PCGs中有4个具有不完整的终止密码子，TA（cob、atp6、nd3）或T（cox3）。[物种名称]线粒体基因组的A+T含量（线粒体DNA-I为70.2%，线粒体DNA II为70.4%）明显高于[另一物种名称]线粒体基因组（线粒体DNA-I为63.9%，线粒体DNA-II为62.9%）。
