Brake Mohammad H, Migdadi Hussein M, Sadder Monther T, Awabdeh Sami, Jawasreh Khaleel, Obeidat Wisam, Al Omari Ruba, Haddad Nizar J
Science Faculty, Jerash University, Jerash, Jordan.
National Agricultural Research Center - NARC, Baqa'a, Jordan.
Mitochondrial DNA B Resour. 2021 Mar 26;6(3):1263-1264. doi: 10.1080/23802359.2021.1906179.
Using high-throughput sequencing technology, the complete mitochondrial genome of Awassi-Jo breed () was decoded. Mitochondrial genome was 16,617 bp in length. The genome contained 37 genes (13 protein-coding, 22 tRNA, and 2 rRNA) and a control region (D-loop region). The genes were encoded on the H-strand, except for the ND6 gene and 8 tRNA genes, which were encoded on the L-strand. The GC content is 38.9%. Phylogenetic analysis was performed to compare Awassi-Jo with other sheep breeds. The phylogenetic tree showed that Awassi-Jo diverged earlier than related breeds (Turkey, Italy, Germany, and Netherland) with a common ancestor in haplogroup HB. The results revealed the importance of mitochondrial data in studying sheep evolution and domestication.
利用高通量测序技术，对阿瓦西-乔品种（）的完整线粒体基因组进行了解码。线粒体基因组长度为16,617bp。该基因组包含37个基因（13个蛋白质编码基因、22个tRNA基因和2个rRNA基因）以及一个控制区（D环区）。除了在轻链上编码的ND6基因和8个tRNA基因外，其他基因均在重链上编码。GC含量为38.9%。进行了系统发育分析，以比较阿瓦西-乔与其他绵羊品种。系统发育树表明，阿瓦西-乔在单倍群HB中与相关品种（土耳其、意大利、德国和荷兰）相比分化更早，拥有一个共同祖先。结果揭示了线粒体数据在研究绵羊进化和驯化中的重要性。
