Section for Food Safety and Animal Health Research, Department of Animal Health, Welfare and Food Safety, Norwegian Veterinary Institute, Oslo, Norway.
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
J Antimicrob Chemother. 2021 Jul 15;76(8):2012-2016. doi: 10.1093/jac/dkab118.
To define characteristics of Klebsiella pneumoniae complex (hereafter KP) isolates from healthy pigs, farm workers and their household members in Thailand.
A total of 839 individual rectal swabs from pigs on 164 farms and 271 faecal samples of humans working on pig farms and persons living in the same household in Khon Kaen, Thailand were screened for gut colonization by KP. Genomic sequences were investigated for antibiotic resistance and virulence genes. Phylogenetic analyses were performed in addition to comparison with isolates from previous studies from Thailand.
KP was detected in approximately 50% of pig and human samples. In total, 253 KP isolates were obtained: 39% from pigs, 34% from farmers and 26% from individuals living on the same farm but without animal contact. MLST revealed high genetic diversity with 196 different STs distributed over four phylogroups (Kp1 to Kp4). Low prevalence of ESBL-KP (7.5%) and colistin-resistant KP (3.2%) was observed among pigs and humans. Remarkably, four convergent MDR and hypervirulent strains were observed: one from pigs (ST290) and three from humans [ST35, ST3415 (strain 90CP1), ST17 (strain 90CM2)]. Sharing of KP clones among pigs and humans was identified for some STs including ST4788, ST661, ST3541 and ST29.
The study indicated a low prevalence of ESBL and mcr genes among KP isolated from pigs and healthy humans in Thailand and suggested the possibility of zoonotic transmission for a subset of circulating KP clones.
定义泰国健康猪、农场工人及其家庭成员中产气肠杆菌复合体（以下简称 KP）分离株的特征。
共采集了泰国孔敬府 164 个农场的 839 份猪直肠拭子和 271 份在养猪场工作的人和与农场工人同住的人的粪便样本，以筛选 KP 肠道定植。对基因组序列进行了抗生素耐药性和毒力基因检测。此外，还进行了系统发育分析，并与来自泰国以前研究的分离株进行了比较。
KP 在大约 50%的猪和人样本中被检测到。共获得 253 株 KP 分离株：39%来自猪，34%来自农民，26%来自同一农场但无动物接触的个体。MLST 显示出很高的遗传多样性，有 196 个不同的 ST 分布在四个 phylogroups（Kp1 至 Kp4）中。在猪和人群中，ESBL-KP（7.5%）和多粘菌素耐药性 KP（3.2%）的流行率较低。值得注意的是，观察到四个趋同的 MDR 和高毒力菌株：一个来自猪（ST290），三个来自人[ST35、ST3415（菌株 90CP1）、ST17（菌株 90CM2）]。一些 ST 包括 ST4788、ST661、ST3541 和 ST29 等，在猪和人群之间发现了 KP 克隆的共享。
该研究表明，泰国猪和健康人群中分离的 KP 中 ESBL 和 mcr 基因的流行率较低，并表明一些流行的 KP 克隆可能存在人畜共患传播的可能性。
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