Pierce S T, Bishop A K, Thompson J M
Department of Anatomical Sciences, University of Illinois, Urbana 61801.
Brain Res. 1988 May 16;468(2):213-22. doi: 10.1016/0165-3806(88)90133-2.
It has been previously found that neurite outgrowth on collagen substrates decreases with increasing gestational age of chick embryo spinal cord and retinal neurons in tissue culture. In the current study, laminin, polylysine and collagen were compared in their efficacy in promoting neurite extension from chick embryo spinal cord neurons aged 6-16 days or retinal neurons aged 8-16 days in ovo. The percentage of neurons with neurites and the length of the neurites were determined at 1 and 3 days in culture. There was a significant increase in neuritogenesis by laminin and polylysine compared to collagen for both spinal cord and retinal neurons. Further, in spinal cord cultures grown on a laminin substrate, there was no decline in neurite outgrowth with increasing developmental age of the neurons as was seen on collagen and polylysine. Neurite length measurements also demonstrated a significant stimulation of neuritogenesis for spinal cord, but not retinal, neurons by laminin compared to polylysine or collagen in 1-day cultures. The results demonstrate tissue-specific differences in the developmental patterns of neurite outgrowth. Retinal neurons appear to have intrinsic changes in their ability to respond to extracellular promoting factors or substrates, while spinal cord neurite outgrowth can be regulated by these extrinsic factors.
先前已经发现，在组织培养中，鸡胚脊髓和视网膜神经元在胶原蛋白底物上的神经突生长会随着鸡胚胎龄的增加而减少。在当前研究中，对层粘连蛋白、聚赖氨酸和胶原蛋白促进鸡胚6至16日龄脊髓神经元或8至16日龄视网膜神经元在卵内神经突延伸的效果进行了比较。在培养1天和3天时测定有神经突的神经元百分比和神经突长度。与胶原蛋白相比，层粘连蛋白和聚赖氨酸对脊髓和视网膜神经元的神经突形成均有显著增加。此外，在层粘连蛋白底物上生长的脊髓培养物中，神经突生长不会像在胶原蛋白和聚赖氨酸上那样随着神经元发育年龄的增加而减少。神经突长度测量还表明，在1天的培养中，与聚赖氨酸或胶原蛋白相比，层粘连蛋白对脊髓神经元的神经突形成有显著刺激作用，但对视网膜神经元没有。结果表明神经突生长发育模式存在组织特异性差异。视网膜神经元对细胞外促进因子或底物的反应能力似乎有内在变化，而脊髓神经突生长可受这些外在因素调节。
