Rousseau-Merck M F, de Keyzer Y, Bourdeau A, Cournot G, Mercier F, Nezelof C
Pathologie Pédiatrique Hôpital Necker, Paris, France.
Cancer. 1988 Jul 15;62(2):303-8. doi: 10.1002/1097-0142(19880715)62:2<303::aid-cncr2820620213>3.0.co;2-k.
The ability of infantile hypercalcemic tumors (three rhabdoid renal tumors, one cellular mesoblastic nephroma, and one hepatoblastoma) to produce parathyroid hormone (PTH) was tested using RNA-DNA hybridization. Results were compared with those obtained in one lung epidermoid carcinoma and one parathyroid adenoma from adult patients. Elevated plasma immunoreactive PTH (iPTH) concentrations were observed in three of five children. The only tumor in which PTH-RNA hybridization could be detected was the parathyroid adenoma. The integrity of the RNA preparations was further confirmed by positive hybridization obtained with a glucagon DNA probe in both normal pancreas and the rhabdoid tumors. Quantitative bone histomorphometry of tumor-bearing nude mice showed a reduction in bone formation and increased bone resorption, the opposite of what occurs in hyperparathyroidism. The PTH-like protein, which was detected by radioimmunoassays (RIA) in the sera of three patients, could not be correlated with tumor PTH mRNA transcription within the limits of our assays. In order to explain this discrepancy, we suggest that the tumors produce a factor (not PTH) which, in turn, elicits the excess iPTH which we detected by RIA.
采用RNA-DNA杂交技术检测了婴儿高钙血症肿瘤（3例横纹肌样肾肿瘤、1例细胞性中胚叶肾瘤和1例肝母细胞瘤）产生甲状旁腺激素（PTH）的能力。将结果与1例成年患者的肺表皮样癌和1例甲状旁腺腺瘤的检测结果进行了比较。在5名儿童中有3名观察到血浆免疫反应性PTH（iPTH）浓度升高。唯一能检测到PTH-RNA杂交的肿瘤是甲状旁腺腺瘤。用胰高血糖素DNA探针在正常胰腺和横纹肌样肿瘤中均获得阳性杂交结果，进一步证实了RNA制剂的完整性。对荷瘤裸鼠进行的定量骨组织形态计量学显示，骨形成减少，骨吸收增加，这与甲状旁腺功能亢进时的情况相反。在3例患者血清中通过放射免疫分析（RIA）检测到的PTH样蛋白，在我们的检测范围内与肿瘤PTH mRNA转录无关。为了解释这种差异，我们认为肿瘤产生一种因子（而非PTH），该因子进而引发我们通过RIA检测到的过量iPTH。
