College of Chemistry, Jilin University, Changchun, 130012, China.
College of Chemistry, Jilin University, Changchun, 130012, China.
Anal Chim Acta. 2021 May 1;1157:338383. doi: 10.1016/j.aca.2021.338383. Epub 2021 Mar 12.
Protein phosphorylation regulates the conformations and function of proteins, which plays an important part in organisms. However, systematic and in-depth analysis of phosphorylation often hinders on account of the low abundance and suppressed ionization of phosphopeptides. Various materials based on single enrichment mechanism show potential in phosphopeptides enrichment, but the enrichment performance is typically not satisfactory. Herein, we developed a carnosine (Car) functionalized magnetic metal organic framework designed as FeO@NH@ZIF-90@Car. Benefiting from the multiple recognition groups of Car and massive metal ions site of ZIF-90, the as-fabricated FeO@NH@ZIF-90@Car was utilized as a multifunctional material with synergistic effect for phosphopeptides enrichment. On the basis of combined immobilized metal ion affinity chromatography (IMAC) and amine-based affinity enrichment mechanism, FeO@NH@ZIF-90@Car exhibited higher enrichment performance of phosphopeptides compared with FeO@NH@ZIF-90 (single IMAC mechanism). Besides, the feasibility of FeO@NH@ZIF-90@Car nanocomposites in complicated samples was further verified by enriching phosphopeptides from nonfat milk, human fluids such as serum and saliva, demonstrating its bright application prospects in phosphoproteomics analysis.
蛋白质磷酸化调节蛋白质的构象和功能，在生物体中起着重要作用。然而，由于磷酸肽的丰度低和离子化抑制，对磷酸化的系统和深入分析常常受到阻碍。各种基于单一富集机制的材料在磷酸肽富集方面显示出潜力，但富集性能通常并不理想。本文开发了一种基于肉碱（Car）功能化的磁性金属有机骨架，设计为 FeO@NH@ZIF-90@Car。得益于 Car 的多种识别基团和 ZIF-90 的大量金属离子位，所制备的 FeO@NH@ZIF-90@Car 被用作具有协同效应的多功能材料，用于磷酸肽的富集。基于固载化金属离子亲和色谱（IMAC）和基于胺的亲和富集机制，FeO@NH@ZIF-90@Car 表现出比 FeO@NH@ZIF-90（单一 IMAC 机制）更高的磷酸肽富集性能。此外，通过从非脂乳、血清和唾液等人体液中富集磷酸肽，进一步验证了 FeO@NH@ZIF-90@Car 纳米复合材料在复杂样品中的可行性，表明其在磷酸蛋白质组学分析中具有广阔的应用前景。
