Inner Mongolia Engineering Research Centre of Lithium-Sulfur Battery Energy Storage, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), College of Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao, 028000, PR China.
Anal Bioanal Chem. 2024 Aug;416(19):4289-4299. doi: 10.1007/s00216-024-05365-y. Epub 2024 Jun 5.
The reasonable design of metal-organic framework (MOF)-derived nanomaterial has important meaning in increasing the enrichment efficiency in the study of protein phosphorylation. In this work, a polyoxometalate (POM) functionalized magnetic MOF nanomaterial (FeO@MIL-125-POM) was designed and fabricated. The nanomaterial with multi-affinity sites (unsaturated metal sites and metal oxide clusters) was used for the enrichment of phosphopeptides. FeO@MIL-125-POM had high-efficient enrichment performance towards phosphopeptides (selectivity, a mass ratio of bovine serum albumin/α-casein/β-casein at 5000:1:1; sensitivity, 0.1 fmol; satisfactory repeatability, ten times). Furthermore, FeO@MIL-125-POM was employed to enrich phosphopeptides from non-fat milk digests, saliva, serum, and A549 cell lysate. The enrichment results illustrated the great potential of FeO@MIL-125-POM for efficient identification of low-abundance phosphopeptides.
合理设计金属-有机骨架（MOF）衍生纳米材料对于提高蛋白质磷酸化研究中的富集效率具有重要意义。本工作设计并制备了一种多酸（POM）功能化磁性 MOF 纳米材料（FeO@MIL-125-POM）。该纳米材料具有多亲和位点（不饱和金属位点和金属氧化物簇），可用于磷肽的富集。FeO@MIL-125-POM 对磷肽具有高效的富集性能（选择性，牛血清白蛋白/α-酪蛋白/β-酪蛋白的质量比为 5000:1:1；灵敏度，0.1 fmol；良好的重现性，重复 10 次）。此外，FeO@MIL-125-POM 还用于从脱脂牛奶消化物、唾液、血清和 A549 细胞裂解物中富集磷肽。富集结果表明，FeO@MIL-125-POM 具有高效鉴定低丰度磷肽的巨大潜力。
