Life Sciences Department, College of Science and literature Mahyel Aseer, King Khalid University, Abha, Saudi Arabia/Unit of Food Bacteriology, Central Laboratory of Food Hygiene, Ministry of Health, Branch in Zagazig, Sharkia, Egypt.
Department of Biology, College of Science, King Khalid University, Abha, Kingdom of Saudi Arabia (KSA)/Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena, Egypt.
Pak J Pharm Sci. 2020 Sep;33(5(Supplementary)):2285-2291.
Silver nanoparticles were synthesized using extra virgin olive oil (Olea europaea L.) and sunflower oil (Helianthus annuus L.) and characterized by UV-vis spectroscopy, X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). The brown color solution of olive oil nanoparticles (EVOO-NPs) and sunflower oil nanoparticles (SFO-NPs) showed typical absorption at 418 nm and 434 nm respectively. The morphology of extra virgin olive oil was found to be in semi cubic shapes with particle size of 23.45 nm (XRD) and 42.30 nm (SEM) while particle size of (SFO-NPs) had 42.30 nm (XRD) and 46.80 nm (SEM). Antimicrobial activities of crude extra virgin olive oil (EVOO), crude sunflower oil (SFO), synthesized nanoparticle from (EVOO-NPs) and (SFO-NPs) against human pathogenic strains were investigated. Synthesized nanoparticle from each oil showed a potent antimicrobial activity against all tested micro-organisms than crude oil which increased by (81.14% to 174.65 %) and by (111.65% to 192.31 %) than (EVOO) and (SFO) respectively. Both (EVOO-NPs) and (EVOO) had more antimicrobial activities than (SFO-NPs) and (SFO). EVOO (NPs) and SFO (NPs) showed maximum antibacterial activities against K. pneumoniae. Therefore (EVOO-NPs) and (SFO-NPs) could be used as safe natural product against multidrug resistant microbes.
使用特级初榨橄榄油（Olea europaea L.）和葵花籽油（Helianthus annuus L.）合成了银纳米粒子，并通过紫外可见光谱、X 射线粉末衍射（XRD）和扫描电子显微镜（SEM）进行了表征。橄榄油纳米粒子（EVOO-NPs）和葵花籽油纳米粒子（SFO-NPs）的棕色溶液分别在 418nm 和 434nm 处显示出典型的吸收峰。特级初榨橄榄油的形态被发现为半立方形状，粒径分别为 23.45nm（XRD）和 42.30nm（SEM），而（SFO-NPs）的粒径为 42.30nm（XRD）和 46.80nm（SEM）。研究了粗制特级初榨橄榄油（EVOO）、粗制葵花籽油（SFO）、合成的 EVOO-NPs 和 SFO-NPs 对人体致病菌株的抗菌活性。与粗油相比，合成的纳米粒子从每种油中显示出对所有测试微生物的强大抗菌活性，增加了 81.14%至 174.65%，而比 EVOO 和 SFO 分别增加了 111.65%至 192.31%。EVOO-NPs 和 EVOO 都比 SFO-NPs 和 SFO 具有更强的抗菌活性。EVOO（NPs）和 SFO（NPs）对肺炎克雷伯菌表现出最大的抗菌活性。因此，EVOO-NPs 和 SFO-NPs 可作为安全的天然产物用于对抗多药耐药微生物。
