Malejczyk J, Moskalewski S
Department of Histology and Embryology, Warsaw Medical School, Poland.
Clin Orthop Relat Res. 1988 Jul(232):292-303.
Strong short-term immunosuppression improved survival of cartilage formed by transplanted allogeneic epiphyseal chondrocytes in mice. The agents tested were cortisone acetate (CA), cyclophosphamide (CY), procarbazine (PCH), and antithymocyte serum (ATS). Their effect on syngeneic grafts was examined morphologically and histomorphometrically. In untreated recipients, chondrocytes formed cartilage nodules that underwent endochondral ossification. Except for high repetitive doses of CY, none of the other agents interfered with normal cartilage formation. However, all agents affected endochondral ossification. In the allogeneic system, the effect of immunosuppression was examined morphologically and by evaluation of specific humoral and cellular antigraft immunity. Allogeneic chondrocytes evoked a strong immune response in untreated mice, and cartilage was gradually destroyed by infiltrating cells. Endochondral ossification did not occur in this system. Neither agent given alone exerted a marked, long-lasting protective effect upon the graft. However, combined treatment with ATS and PCH inhibited immune response and completely prevented infiltrate formation and allowed endochondral ossification similar to that in the syngeneic control. Although some weak signs of antigraft immunity were seen after six weeks, it is possible that they were due to secondary exposure of antigen-bearing chondrocytes in the course of endochondral ossification.
强效短期免疫抑制可提高小鼠同种异体骨骺软骨细胞移植形成的软骨的存活率。所测试的药物有醋酸可的松（CA）、环磷酰胺（CY）、丙卡巴肼（PCH）和抗胸腺细胞血清（ATS）。通过形态学和组织形态计量学检查了它们对同基因移植物的影响。在未治疗的受体中，软骨细胞形成软骨结节并经历软骨内成骨。除了高重复剂量的CY外，其他药物均未干扰正常软骨形成。然而，所有药物均影响软骨内成骨。在同种异体系统中，通过形态学以及评估特异性体液和细胞抗移植物免疫来检查免疫抑制的效果。同种异体软骨细胞在未治疗的小鼠中引发强烈的免疫反应，软骨被浸润细胞逐渐破坏。该系统中未发生软骨内成骨。单独给予任何一种药物对移植物均未产生显著的、持久的保护作用。然而，ATS和PCH联合治疗可抑制免疫反应，完全防止浸润形成，并使软骨内成骨类似于同基因对照组。尽管六周后出现了一些微弱的抗移植物免疫迹象，但有可能是由于软骨内成骨过程中含抗原软骨细胞的二次暴露所致。
