Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos, Pampulha, Belo Horizonte, MG, 31270-901, Brazil.
Department of Invertebrates, Natural History Museum, P.O. Box 6434, 6, CH-1211, Geneva, Switzerland.
Parasitol Res. 2021 May;120(5):1593-1603. doi: 10.1007/s00436-021-07138-3. Epub 2021 Apr 9.
Tapeworms (Cestoda: Proteocephalidae) are the dominant component of communities of intestinal parasites in pimelodid and other catfishes (Siluriformes) from South America. Even though these parasites have been studied intensively over more than one century, molecular taxonomy and phylogenetics have questioned their morphology-based classification, thus raising doubts about the systematic value of traits commonly used to circumscribe individual taxa. In the present study, members of three morphologically well-characterized genera of proteocephalids from pimelodid (Hemisorubim platyrhynchos and Sorubim lima) and auchenipterid (Ageneiosus inermis) catfishes from the Paraná or Amazon River basins were subjected to DNA sequencing of the large subunit nuclear ribosomal RNA (lsrDNA) and complete mitochondrial cytochrome c oxidase subunit I (COI). Phylogenetic analyses revealed the sister relationship between Manaosia bracodemoca and Mariauxiella piscatorum, and among Mariauxiella pimelodi and Ageneiella brevifilis. As a result, Mar. piscatorum and A. brevifilis are transferred to Manaosia and Mariauxiella, respectively, as Manaosia piscatorum n. comb. and Mariauxiella brevifilis n. comb., and the genus Ageneiella is suppressed. Diagnoses of Manaosia and Mariauxiella are amended. In addition, the present study revealed misidentification of tapeworms whose sequences are deposited in the GenBank database.
带绦虫（Cestoda：Proteocephalidae）是南美的皮米洛德鱼和其他鲶形目鱼类（Siluriformes）肠道寄生虫群落的主要组成部分。尽管这些寄生虫已经被研究了一个多世纪，但分子分类学和系统发生学对其基于形态的分类提出了质疑，从而对用于划定个别分类群的特征的系统学价值产生了怀疑。在本研究中，来自巴拉那河或亚马逊河流域的皮米洛德鱼（Hemisorubim platyrhynchos 和 Sorubim lima）和 Auchenipteridae 鲶鱼（Ageneiosus inermis）的三种形态特征良好的 Proteocephalidae 成员接受了大核核糖体 RNA（lsrDNA）和完整线粒体细胞色素 c 氧化酶亚基 I（COI）的 DNA 测序。系统发育分析显示了 Manaosia bracodemoca 和 Mariauxiella piscatorum 之间以及 Mariauxiella pimelodi 和 Ageneiella brevifilis 之间的姐妹关系。结果，Mar. piscatorum 和 A. brevifilis 分别转移到 Manaosia 和 Mariauxiella 中，作为 Manaosia piscatorum n. comb. 和 Mariauxiella brevifilis n. comb.，并抑制了 Ageneiella 属。修改了 Manaosia 和 Mariauxiella 的诊断。此外，本研究还揭示了在 GenBank 数据库中存储的序列被错误鉴定的带绦虫。
