Forensic DNA Division, National Forensic Service, 10, Ipchun-ro, Wonju si., Gangwon-do 26460, Republic of Korea.
Department of Forensic Sciences, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu Suwon-si, Gyeongi-do 16419, Republic of Korea.
Leg Med (Tokyo). 2021 Jul;51:101872. doi: 10.1016/j.legalmed.2021.101872. Epub 2021 Apr 1.
Recently, a method of identifying body fluids using DNA methylation has been developed (Frumkin et al., 2011). An existing multiplex assay using 9 CpG markers could differentiate 5 body fluids: semen, blood, saliva, menstrual blood, and vaginal fluid. To validate this technique, we evaluated the previously described body fluid identification method by means of single base extension (SBE). DNA methylation was applied to 22 samples in 18 forensic cases; seven of these were semen, three were blood, eight were saliva, three were vaginal fluid, and one was menstrual blood. Total of 18 samples were tested, the DNA methylation profiles were coincident from preliminary tests (acid phosphatase (AP), leucomalachite green (LMG, Sigma Aldrich, St Louis, MO, USA) and SALIgAE®) except one sample which displayed an all-negative result. After applying the DNA methylation method to forensic samples, we determined that it could be very useful for differentiating vaginal secretions from menstrual blood, for which there is no conventional preliminary testing method.
最近，一种使用 DNA 甲基化识别体液的方法已经被开发出来（Frumkin 等人，2011 年）。一种现有的使用 9 个 CpG 标记物的多重分析可以区分 5 种体液：精液、血液、唾液、月经血和阴道分泌物。为了验证这项技术，我们通过单碱基延伸（SBE）对先前描述的体液识别方法进行了评估。DNA 甲基化应用于 18 个法医案例中的 22 个样本；其中 7 个是精液，3 个是血液，8 个是唾液，3 个是阴道分泌物，1 个是月经血。总共测试了 18 个样本，除了一个样本显示全阴性结果外，初步测试（酸性磷酸酶（AP）、亮绿（LMG，Sigma Aldrich，St Louis，MO，USA）和 SALIgAE®）的 DNA 甲基化图谱是一致的。在将 DNA 甲基化方法应用于法医样本后，我们确定它对于区分阴道分泌物和月经血非常有用，因为目前没有常规的初步测试方法。
