Safford R, Windust J H, Lucas C, De Silva J, James C M, Hellyer A, Smith C G, Slabas A R, Hughes S G
Biosciences Division, Unilever Research Laboratory, Sharnbrook, Bedfordshire, England.
Eur J Biochem. 1988 Jun 1;174(2):287-95. doi: 10.1111/j.1432-1033.1988.tb14096.x.
Acyl-carrier protein (ACP) is a key component involved in the regulation of fatty acid biosynthesis in plants. cDNA clones encoding ACP from Brassica napus (oil seed rape) embryos have been isolated using oligonucleotide probes derived from heterologous ACPs. Analysis of the DNA sequence data, in conjunction with N-terminal amino acid sequence data, revealed ACP to be synthesized from nuclear DNA as a precursor containing a 51-amino-acid N-terminal extension. Immunocytochemical studies showed ACP to be localised solely within the plastids of B. napus seed tissue and it would therefore appear that the N-terminal extension functions as a transit peptide to direct ACP into these organelles. Analysis of several cDNA clones revealed sequence heterogeneity and thus evidence for an ACP multigene family. From ten cDNA clones, six unique genes, encoding five different mature ACP polypeptides, were identified. Northern blot hybridisation studies provide evidence that the seed and leaf forms of rape ACP are encoded by structurally distinct gene sets.
酰基载体蛋白（ACP）是参与植物脂肪酸生物合成调控的关键成分。利用源自异源ACP的寡核苷酸探针，已从甘蓝型油菜（油菜籽）胚胎中分离出编码ACP的cDNA克隆。结合N端氨基酸序列数据对DNA序列数据进行分析，结果显示ACP由核DNA合成，作为一种含有51个氨基酸N端延伸的前体。免疫细胞化学研究表明，ACP仅定位于甘蓝型油菜种子组织的质体中，因此N端延伸似乎起着转运肽的作用，将ACP导入这些细胞器。对几个cDNA克隆的分析揭示了序列异质性，从而证明存在一个ACP多基因家族。从十个cDNA克隆中，鉴定出六个独特基因，它们编码五种不同的成熟ACP多肽。Northern印迹杂交研究提供了证据，表明油菜ACP的种子和叶片形式由结构不同的基因集编码。
