基于 RAD-seq 的毛鳞鱼（Setipinna tenuifilis）大规模 SNP 数据集的鉴定。
Identification of a large dataset of SNPs in hair-fin anchovy (Setipinna tenuifilis) based on RAD-seq.
机构信息
National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Zhejiang Ocean University, No.1 Haidanan road, Zhoushan, 316022, China.
National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, No.1 Haidanan road, Zhoushan, 316022, China.
出版信息
Anim Genet. 2021 Jun;52(3):371-374. doi: 10.1111/age.13062. Epub 2021 Apr 11.
Hair-fin anchovy (Setipinna tenuifilis) is an economically important fish distributed in the West Indian Ocean and the Northwest Pacific Ocean. In this study, 154 individuals in eight populations of S. tenuifilis were sequenced and 850 million raw reads were obtained using restriction site-associated DNA sequencing (RAD-seq). First, we identified 14 012 044 hypothetical SNP markers. A dataset of 199 903 high-quality SNPs was collected after further screening. These SNPs have a strong ability to test the genetic diversity between the eight populations. The differentiation and genetic law between samples were explored based on SNPs in populations of S. tenuifilis. The results of this study will provide data for protecting the genetic resources of the species.
鳀鱼（Setipinna tenuifilis）是一种经济价值很高的鱼类，分布于西印度洋和西北太平洋。本研究采用 RAD-seq 技术对 8 个鳀鱼群体的 154 个个体进行测序，共获得 8.5 亿个原始reads。首先，我们鉴定了 14012044 个假设 SNP 标记。进一步筛选后，获得了一个包含 199903 个高质量 SNP 的数据集。这些 SNP 具有较强的能力来检验 8 个鳀鱼群体之间的遗传多样性。基于鳀鱼种群中的 SNP，对样本间的分化和遗传规律进行了探索。本研究结果将为保护该物种的遗传资源提供数据支持。
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