高果糖饮食诱导的 Scd1 基因在大鼠肝脏中的表达与组蛋白 H3 和 H4 的乙酰化增加以及 ChREBP 在 Scd1 启动子上的结合有关。
High-fructose diet-induced hepatic expression of the Scd1 gene is associated with increased acetylation of histones H3 and H4 and the binding of ChREBP at the Scd1 promoter in rats.
机构信息
Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University.
Department of Life Science and Chemistry, Graduate School of Natural Science and Technology, Gifu University.
出版信息
Biomed Res. 2021;42(2):85-88. doi: 10.2220/biomedres.42.85.
Stearoyl-CoA desaturase-1 (SCD1) is a key enzyme in the biosynthesis of monounsaturated fatty acids, and the expression of the Scd1 gene is induced by the intake of the lipogenic sugar fructose. We examined the effects of a high-fructose diet on hepatic acetylation of histones H3 and H4 and the binding of carbohydrate response element-binding protein (ChREBP) on the Scd1 gene promoter in rats. Rats were fed a control diet or a high-fructose diet for 10 days. The intake of a high-fructose diet significantly increased histone H3 and H4 acetylation and ChREBP binding to the Scd1 gene promoter as well as the amount of triglyceride and the expression of the Scd1 gene. These results suggest that short-term intake of high fructose upregulates expression of Scd1 by enhancing acetylation of histones H3 and H4 and binding of ChREBP at the Scd1 promoter.
硬脂酰辅酶 A 去饱和酶 1（SCD1）是单不饱和脂肪酸生物合成中的关键酶，Scd1 基因的表达受摄入的生糖性糖果糖的诱导。我们研究了高果糖饮食对大鼠肝组织组蛋白 H3 和 H4 乙酰化以及碳水化合物反应元件结合蛋白（ChREBP）与 Scd1 基因启动子结合的影响。大鼠连续 10 天给予对照饮食或高果糖饮食。高果糖饮食显著增加了组蛋白 H3 和 H4 的乙酰化以及 ChREBP 与 Scd1 基因启动子的结合，同时也增加了甘油三酯的含量和 Scd1 基因的表达。这些结果表明，短期摄入高果糖可通过增强组蛋白 H3 和 H4 的乙酰化以及 ChREBP 与 Scd1 启动子的结合来上调 Scd1 的表达。
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