在体外眨眼模拟眼模型与静态小瓶沉积模型中，溶菌酶在隐形眼镜上的沉积。

Lysozyme Deposition on Contact Lenses in an In Vitro Blink-Simulation Eye Model Versus a Static Vial Deposition Model.

机构信息

Centre for Ocular Research and Education (CORE) (V.W.Y.C., C.-M.P., W.N., L.J.), School of Optometry and Vision Science, University of Waterloo, Waterloo, ON, Canada ; and Centre for Eye and Vision Research (CEVR) (C.-M.P., W.N., L.J.), Hong Kong, China .

出版信息

Eye Contact Lens. 2021 Jul 1;47(7):388-393. doi: 10.1097/ICL.0000000000000784.

DOI:10.1097/ICL.0000000000000784

PMID:33840748

Abstract

PURPOSE

To evaluate active lysozyme deposition on daily disposable (DD) contact lenses (CL) using a novel in vitro blink model.

METHODS

Three conventional hydrogel DD CL materials (etafilcon A, omafilcon A, nelfilcon A) and three silicone hydrogel DD CL materials (delefilcon A, senofilcon A, somofilcon A) were tested. The device blink rate was set to 6 blinks/min with a tear flow rate of 1 μL/min using an artificial tear solution (ATS) containing lysozyme and other typical tear film components. After incubation at 2, 4, or 8 hr, lenses were removed, and lysozyme activity was measured. A separate experiment was conducted with lenses incubated in a static vial containing 480 μL of ATS.

RESULTS

Etafilcon A deposited significantly higher amounts of active lysozyme (402±102 μg/lens) than other lens materials after 8 hr (P<0.0001). Etafilcon A had a higher amount of active lysozyme using the blink model compared with the static vial (P=0.0435), whereas somofilcon A (P=0.0076) and senofilcon A (P=0.0019) had a higher amount of lysozyme activity in the vial compared with the blink model.

CONCLUSION

The blink model can be tuned to provide quantitative data that closely mimics ex vivo studies and can be used to model deposition of lysozyme on CL materials.

摘要

目的

使用新型体外眨眼模型评估日戴型（DD）隐形眼镜（CL）上的活性溶菌酶沉积。

方法

测试了三种常规水凝胶 DD CL 材料（etafilcon A、 omafilcon A、 nelfilcon A）和三种硅水凝胶 DD CL 材料（delefilcon A、 senofilcon A、 somofilcon A）。使用含有溶菌酶和其他典型泪膜成分的人工泪液（ATS）将设备眨眼频率设置为 6 次/分钟，泪液流速为 1 μL/min。在 2、4 或 8 小时孵育后，取出镜片并测量溶菌酶活性。在含有 480 μL ATS 的静态小瓶中孵育镜片的单独实验。

结果

在 8 小时后，etafilcon A 沉积的活性溶菌酶量（402±102 μg/镜片）明显高于其他镜片材料（P<0.0001）。与静态小瓶相比，眨眼模型下的 etafilcon A 具有更高的活性溶菌酶量（P=0.0435），而 somofilcon A（P=0.0076）和 senofilcon A（P=0.0019）在小瓶中的溶菌酶活性高于眨眼模型。