Hunt L T, Barker W C
National Biomedical Research Foundation, Georgetown University Medical Center, Washington, DC 20007.
FEBS Lett. 1988 Jun 20;233(2):282-8. doi: 10.1016/0014-5793(88)80443-5.
We observed a striking sequence similarity between precursors for promagainin and procaerulein type I (excluding the caerulein peptide region). Additional comparisons of the promagainin precursor with those of other procaeruleins, proxenopsin, and peptide-Gly-Leu-amide revealed that all possess one or more copies of a structurally similar spacer module, from which an amphiphilic spacer peptide is cleaved. Promagainin yields the magainins, spacer peptides with antimicrobial activity; we suggest other spacer peptides may have similar activity. We propose that the genes for the four kinds of hormones were derived from a common ancestral gene through gene and exon duplications and that the procaerulein and proxenopsin genes are mosaic genes in which the original 3'-ends were replaced by exon shuffling.
我们观察到前蛙皮素原和I型前铃蟾肽原（不包括铃蟾肽肽区）之间存在显著的序列相似性。将前蛙皮素原与其他前铃蟾肽、前 Xenopsin和肽 - 甘氨酸 - 亮氨酸 - 酰胺的前体进行的其他比较表明，它们都拥有一个或多个结构相似的间隔模块拷贝，从中切割出两亲性间隔肽。前蛙皮素产生具有抗菌活性的蛙皮素间隔肽；我们认为其他间隔肽可能具有类似活性。我们提出，这四种激素的基因是通过基因和外显子重复从一个共同的祖先基因衍生而来的，并且前铃蟾肽和前 Xenopsin基因是镶嵌基因，其中原始的3'末端通过外显子重排被取代。
