大鼠脑中是否存在乙酰胆碱释放蛋白介体？

Is the acetylcholine releasing protein mediatophore present in rat brain?

作者信息

Israël M, Lesbats B, Morel N, Manaranche R, Le Gal la Salle G

机构信息

Département de Neurochimie, CNRS, Gif-sur-Yvette, France.

出版信息

FEBS Lett. 1988 Jun 20;233(2):421-6. doi: 10.1016/0014-5793(88)80475-7.

DOI:10.1016/0014-5793(88)80475-7

PMID:3384100

Abstract

Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatophore activity with properties close to those described for the Torpedo protein (extractability, sensitivity to calcium, and effect of the drug cetiedil). The activity was more abundant in synaptosomal membranes than in mitochondrial or myelinic membranes and in cholinergic areas as compared to cerebellum.

摘要

介质载体是一种从电鳐电器官的神经末梢膜中纯化出来的蛋白质。它赋予人工膜一种依赖钙的机制，可转运乙酰胆碱。当对大鼠脑突触体膜进行类似的重组实验时，发现存在介质载体活性，其特性与所述电鳐蛋白质的特性相近（可提取性、对钙的敏感性以及药物西替地尔的作用）。该活性在突触体膜中比在线粒体膜或髓鞘膜中更丰富，并且与小脑相比，在胆碱能区域中更丰富。