Wolff H, Anderson D J
Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Fertil Steril. 1988 Jul;50(1):129-32.
To evaluate the relationship between concentrations of the granulocytic enzyme polymorphonuclear (PMN) elastase in seminal plasma and the presence of high numbers of white blood cells (WBC) in semen, specimens from 118 infertility patients were analyzed for each variable. PMN-elastase levels were determined by an enzyme-linked immunosorbent assay and WBC numbers by a recently described immunohistologic technique. PMN-elastase levels in seminal plasma correlated well with WBC numbers in semen (r = +0.755). A three-step grading system of PMN-elastase values as low/normal (less than 250 ng/ml), intermediate (250 to 1000 ng/ml) and high/pathologic (greater than 1000 ng/ml) showed a highly significant relationship to a similar WBC number grading system: low/normal, less than 10(5) WBC/ml, intermediate, 10(5) to 10(6) WBC/ml, and high/leukocytospermic, greater than 10(6) WBC/ml semen. Incubation of semen at 37 degrees C resulted in a slight continuous decrease of immunoreactive PMN-elastase at a rate of approximately 10% per 3 hours. As all 18 samples with more than 1000 ng PMN-elastase per ml seminal plasma showed more than 10(6) WBC/ml semen, PMN-elastase levels above 1000 ng/ml were diagnostic for leukocytospermia.
为评估精浆中粒细胞酶多形核（PMN）弹性蛋白酶浓度与精液中大量白细胞（WBC）存在之间的关系，对118例不育患者的样本进行了各变量分析。通过酶联免疫吸附测定法测定PMN - 弹性蛋白酶水平，通过最近描述的免疫组织学技术测定白细胞数量。精浆中的PMN - 弹性蛋白酶水平与精液中的白细胞数量密切相关（r = +0.755）。PMN - 弹性蛋白酶值的三步分级系统为低/正常（低于250 ng/ml）、中等（250至1000 ng/ml）和高/病理（高于1000 ng/ml），与类似的白细胞数量分级系统显示出高度显著的关系：低/正常，低于10⁵白细胞/ml；中等，10⁵至10⁶白细胞/ml；高/白细胞精子症，精液中白细胞高于10⁶/ml。精液在37℃孵育导致免疫反应性PMN - 弹性蛋白酶以每3小时约10%的速率轻微持续下降。由于所有每毫升精浆中PMN - 弹性蛋白酶超过1000 ng的18个样本精液中白细胞均超过10⁶/ml，因此PMN - 弹性蛋白酶水平高于1000 ng/ml可诊断为白细胞精子症。
