Stanislavov R
Akush Ginekol (Sofiia). 1999;38(3):20-1.
The peroxidase method is sufficient for quantification of granulocytes, but immunocytology is the standard for detection of white blood cells (WBC) in semen. Granulocytes are the most prevalent WBC in semen (50 to 60%) followed by macrophages (20 to 30%) and T-lymphocytes (2 to 5%). The frequency of leukocytospermia (> 106 WBC/ml) among male infertility patients is 29%. There are ample evidences for sperm damage by WBC: 1) Seminal WBC numbers were higher in infertile patients than among fertile men; 2) leukocytospermia was associated with decreased sperm numbers and impared sperm motility; 3) WBC damage sperm function and were an important prognostic factor for IVF-ET failure. Approximately 80% of leukocytospermic samples are microbiologically negative. In some cases Chlamydia trachomatis might have triggered a persistent inflammatory reaction. Genital tract inflammation facilitates the formation of sperm antibodies.
过氧化物酶法足以用于粒细胞的定量分析，但免疫细胞学法是检测精液中白细胞（WBC）的标准方法。粒细胞是精液中最常见的白细胞（占50%至60%），其次是巨噬细胞（占20%至30%）和T淋巴细胞（占2%至5%）。男性不育患者中白细胞精子症（>106个白细胞/毫升）的发生率为29%。有充分证据表明白细胞会损害精子：1）不育患者精液中的白细胞数量高于生育男性；2）白细胞精子症与精子数量减少和精子活力受损有关；3）白细胞会损害精子功能，是体外受精-胚胎移植失败的重要预后因素。大约80%的白细胞精子症样本微生物学检测呈阴性。在某些情况下，沙眼衣原体可能引发了持续的炎症反应。生殖道炎症会促进精子抗体的形成。
