Ior Lydia Doosuur, Otimenyin Sunday Oritsetimenyin, Okwuasaba Francis Kanayo
Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Jos, Jos, Nigeria.
IBRO Neurosci Rep. 2021 Jan 22;10:83-89. doi: 10.1016/j.ibneur.2021.01.003. eCollection 2021 Jun.
Psychosis is a chronic neuropsychiatric disorder that affects millions of individuals worldwide and impairs the quality of life and productivity of the patients. Guill & Perr. (Combrataceae) is a plant that is used in the management of anxiety related disorders. The present study investigates the antipsychotic effects of ethyl acetate fraction of (EFTM) leaf in ketamine-induced psychosis in mice. Acute toxicity of EFTM was determine using Lorke's method. Ketamine (25 mg/kg) was injected once daily for 7 consecutive days in Swiss albino mice to induce psychosis. The effect of the extracts (100, 200 and 400 mg/kg) was evaluated against psychotic-like behaviors induced by ketamine including locomotor activity and stereotypy in the open field test, immobility duration in the forced swim test, and memory impairment using the Y- maze test. The acute antipsychotic effect of EFTM was evaluated on apomorphine climbing test, while woodblock test was performed to assess its extrapyramidal side effects. The LD was found to be 3807 mg/kg p.o. which is considered safe. EFTM (100, 200 and 400 mg/kg) exhibited significant antipsychotic effect by reducing ketamine-induced hyperactivity, immobility, and memory deficit in mice, EFTM also suppressed stereotypic climbing behavior due to apomorphine. Accordingly, the antipsychotic activity of EFTM was not associated with extrapyramidal side effects as evidenced by lack of catalepsy. The study revealed that EFTM ameliorated psychotic-like symptoms and is devoid of extrapyramidal side effects in mice, underscoring its antipsychotic-like effect.
精神病是一种慢性神经精神障碍，影响着全球数百万人，损害患者的生活质量和生产力。圭尔&佩尔（使君子科）是一种用于治疗焦虑相关疾病的植物。本研究调查了[植物名称]叶乙酸乙酯部位（EFTM）对氯胺酮诱导的小鼠精神病的抗精神病作用。采用洛克法测定EFTM的急性毒性。在瑞士白化小鼠中连续7天每天注射一次氯胺酮（25mg/kg）以诱导精神病。评估提取物（100、200和400mg/kg）对氯胺酮诱导的类似精神病行为的影响，包括旷场试验中的运动活动和刻板行为、强迫游泳试验中的不动时间，以及使用Y迷宫试验评估记忆损害。在阿扑吗啡攀爬试验中评估EFTM的急性抗精神病作用，同时进行木僵试验以评估其锥体外系副作用。经口LD50为3807mg/kg，被认为是安全的。EFTM（100、200和400mg/kg）通过减少氯胺酮诱导的小鼠多动、不动和记忆缺陷，表现出显著的抗精神病作用，EFTM还抑制了阿扑吗啡引起的刻板攀爬行为。因此，EFTM的抗精神病活性与锥体外系副作用无关，这一点由缺乏僵住症证明。该研究表明，EFTM改善了小鼠类似精神病的症状，且无锥体外系副作用，突出了其类似抗精神病的作用。
