Vaillant C, Le Guellec C, Pakdel F, Valotaire Y
Laboratoire de Biologie Moléculaire, Université de Rennes, France.
Gen Comp Endocrinol. 1988 May;70(2):284-90. doi: 10.1016/0016-6480(88)90148-7.
Using a primary culture of trout hepatocytes we compare the kinetics of accumulation of vitellogenin and its messenger RNA after estrogen administration. We found that the cells were more sensitive to estradiol than to other estrogens. The lowest effective concentration of estradiol was 10(-9) M. At 10(-6) M the androgens have no effect. Comparison of the primary and secondary stimulation with E2 shows that the initial rate of accumulation of vitellogenin is very much higher in the secondary stimulation. Over a time course of primary stimulation we show that after estradiol withdrawal the rate of accumulation of vitellogenin mRNA in the secondary is a function of time of the first stimulation.
利用虹鳟肝细胞原代培养物，我们比较了雌激素给药后卵黄蛋白原及其信使核糖核酸的积累动力学。我们发现，细胞对雌二醇的敏感性高于其他雌激素。雌二醇的最低有效浓度为10^(-9) M。在10^(-6) M时，雄激素无作用。用E2进行初次刺激和二次刺激的比较表明，二次刺激中卵黄蛋白原的初始积累速率要高得多。在初次刺激的时间进程中，我们表明，在撤去雌二醇后，二次刺激中卵黄蛋白原信使核糖核酸的积累速率是第一次刺激时间的函数。
