Department of Chemistry, College of Science, UAE University, Al Ain, UAE.
Department of Chemistry, College of Arts and Sciences, Khalifa University, Abu Dhabi, UAE E-mail:
Water Sci Technol. 2021 Apr;83(7):1535-1547. doi: 10.2166/wst.2021.065.
The accumulation of toxic chemical constituents in sludge and wastewater has fuelled an interest in investigating efficient and eco-friendly wastewater remediation approaches. In this study, a set of bacterial samples were isolated from petroleum sludge and tested for their ability to degrade different aromatic pollutants, including azo dyes and emerging pollutants. Although exhibiting differential specificity, all bacterial isolates were able to degrade different classes of aromatic dyes efficiently. Ribosomal 16S rRNA sequencing of the 12 bacterial isolates showed that they belonged to two different bacterial genera: Bacillus cereus and Pseudomonas guariconensis. Of these 12 strains, MA1 (B. cereus) was the most promising and was chosen for further optimization and biochemical studies. The optimum culture and remediation conditions for MA1 was found to be at pH 7, with 100 ppm dye concentration, and under aerobic condition. In addition to efficiently degrading various aromatic dyes (e.g. Congo Red, Reactive Black 5, PBS, and Toluidine Blue), MA1 was also found to be capable of degrading various emerging pollutants (e.g. prometryn, fluometuron and sulfamethoxazole). Preliminary transcriptome analysis shows that MA1 grown on media containing a mixture of aromatic dyes appears to differentially express a number of genes. Data shown here strongly suggests that petroleum sludge is a rich reservoir of bacteria with powerful remediation abilities.
污泥和废水中有毒化学物质的积累，促使人们研究高效、环保的废水修复方法。本研究从石油污泥中分离出一组细菌样本，并测试它们降解不同芳香族污染物的能力，包括偶氮染料和新兴污染物。尽管具有不同的特异性，但所有细菌分离物都能够有效地降解不同类别的芳香族染料。对 12 株细菌的核糖体 16S rRNA 测序表明，它们属于两个不同的细菌属：蜡状芽孢杆菌和瓜氏假单胞菌。在这 12 株菌中，MA1（蜡状芽孢杆菌）最有前途，因此被选择进行进一步的优化和生化研究。MA1 的最佳培养和修复条件是 pH7、染料浓度 100ppm 和需氧条件。除了能有效降解各种芳香族染料（如刚果红、活性黑 5、PBS 和甲苯胺蓝）外，MA1 还能降解各种新兴污染物（如普灭净、伏草隆和磺胺甲恶唑）。初步的转录组分析表明，MA1 在含有混合芳香族染料的培养基上生长时，似乎会差异表达许多基因。这里的数据强烈表明，石油污泥是具有强大修复能力的细菌的丰富蕴藏库。
