Ando Y, Akaike T, Inoue M, Horisawa Y, Yamada Y, Uekawa K, Fujise R, Araki S
Department of Medicine, Kumamoto University Medical School, Japan.
Hokkaido Igaku Zasshi. 1988 Mar;63(2):207-12.
Iron is one of the essential element for bacterial growth. To study the effect of chelation of iron on bacterial growth, 17 different strains of bacteria were cultured in chocolate-agar medium in the presence or absence of deferoxamine methylate (DM), a potent chelating agent for iron. A fairly low concentration of DM (10 mg/ml) in the culture medium markedly inhibited the growth rates of the three bacterial strains, B. catarrhalis, N. meningiditis and N. gonorrhoeae. In vivo effect of DM was also tested in mice that were intraperitoneally inoculated with a lethal number of N. meningiditis (2 x 10(8)/animal). After 40 h of inoculation, more than 90% of the control animals died for acute peritonitis. In contrast, animals which were intraperitoneally administered with DM showed a marked resistance to the inoculated bacteria without showing toxic effects of the agent: less than 30% of animals died of acute peritonitis caused by infection within 40 h. The results suggest that, in addition to the currently used antibiotics. DM might also be an useful therapeutic agent for treatment of bacterial infection.
铁是细菌生长所必需的元素之一。为研究铁螯合对细菌生长的影响，在有或没有去铁胺甲酸盐（DM，一种有效的铁螯合剂）存在的情况下，将17种不同的细菌菌株在巧克力琼脂培养基中培养。培养基中相当低浓度的DM（10毫克/毫升）显著抑制了三种细菌菌株——卡他莫拉菌、脑膜炎奈瑟菌和淋病奈瑟菌的生长速度。还在小鼠体内测试了DM的作用，这些小鼠经腹腔接种致死剂量的脑膜炎奈瑟菌（2×10⁸/只动物）。接种40小时后，超过90%的对照动物死于急性腹膜炎。相比之下，经腹腔给予DM的动物对接种的细菌表现出显著的抵抗力，且未显示该药物的毒性作用：在40小时内，死于感染引起的急性腹膜炎的动物不到30%。结果表明，除了目前使用的抗生素外，DM可能也是治疗细菌感染的一种有用的治疗剂。
