University of Health Sciences, Antalya Education and Research Hospital, Department of Pathology, Antalya, Turkey.
University of Health Sciences, Antalya Education and Research Hospital, Department of Gynecological Oncology, Antalya, Turkey.
Ginekol Pol. 2021;92(8):544-549. doi: 10.5603/GP.a2021.0016. Epub 2021 Apr 12.
FOXA1 expression has been demonstrated in several hormone-dependent cancers. However, data are limited concerning the role of FOXA1 in endometrial cancers. The present study aimed to investigate FOXA1 expression via the microarray technique in benign hyperplasia, endometrial intraepithelial neoplasia, and endometrial endometrioid carcinoma. We also aimed to determine whether there were any associations between FOXA1 expression, tumor grade, myometrial invasion and lymphatic invasion.
Paraffin-embedded sections prepared from samples obtained from 114 patients who underwent surgical hysterectomy or curettage were analyzed. Data were retrieved from digitally-stored medical records. Tissue microarrays were prepared from formalin-fixed, paraffin-embedded tissue blocks. Full tumor sections were used for immunohistochemical analysis performed.
Carcinomas with nuclear grade 3 had higher FOXA1 values than others, while grade 2 carcinomas also had higher FOXA1 values relative to grade 1 (p < 0.001). FOXA1 values of FIGO stage III carcinomas were significantly higher than others and stage II values were also significantly higher than stage I FOXA1 values (p < 0.001). Patients with myometrial and lymph node invasion had significantly higher FOXA1 values than others (p < 0.001 and p = 0.047, respectively). FOXA1 had 91.30% sensitivity, 63.60% specificity and 77.78% accuracy for predicting the presence of myometrial invasion with a cut-off value of 9.
FOXA1 expression is higher in endometrial endometrioid carcinoma compared to benign endometrial hyperplasia or intraepithelial neoplasia. In patients with endometrial endometrioid carcinoma, high FOXA1 expression is associated with high tumor grade, myometrial and lymph node invasion. However, FOXA1 expression is not associated with lymphovascular or cervical invasion.
FOXA1 的表达已在几种激素依赖性癌症中得到证实。然而，关于 FOXA1 在子宫内膜癌中的作用的数据有限。本研究旨在通过微阵列技术研究良性增生、子宫内膜上皮内瘤变和子宫内膜子宫内膜样癌中的 FOXA1 表达。我们还旨在确定 FOXA1 表达与肿瘤分级、肌层浸润和淋巴血管浸润之间是否存在任何关联。
对 114 例接受子宫切除术或刮宫术的患者的石蜡包埋切片进行分析。数据取自数字存储的病历。从福尔马林固定、石蜡包埋的组织块中制备组织微阵列。对全肿瘤切片进行免疫组织化学分析。
核分级 3 的癌具有较高的 FOXA1 值，而分级 2 的癌相对于分级 1 也具有较高的 FOXA1 值（p<0.001）。FIGO 分期 III 期癌的 FOXA1 值明显高于其他分期，而 II 期值也明显高于 I 期 FOXA1 值（p<0.001）。有肌层和淋巴结浸润的患者具有明显较高的 FOXA1 值（p<0.001 和 p=0.047）。FOXA1 的截断值为 9 时，对预测肌层浸润的敏感性为 91.30%，特异性为 63.60%，准确性为 77.78%。
与良性子宫内膜增生或上皮内瘤变相比，子宫内膜子宫内膜样癌中的 FOXA1 表达更高。在子宫内膜子宫内膜样癌患者中，高 FOXA1 表达与高肿瘤分级、肌层和淋巴结浸润相关。然而，FOXA1 表达与淋巴血管或宫颈浸润无关。
