1Department of Microbiology, Amrita Institute of Medical Sciences & Research Centre, Amrita Vishwa Vidyapeetham, Ponekara, Kochi, Kerala, India.
2Department of Ophthalmology, Little Flower Hospital and Research Centre, Angamaly, Kerala, India.
Am J Trop Med Hyg. 2021 Apr 12;104(6):2046-2049. doi: 10.4269/ajtmh.20-1521.
We tried to determine the epidemiology and species of human dirofilariasis observed at two tertiary care hospitals in Kerala. We searched the hospital database to identify cases of dirofilariosis from January 2005 to March 2020. Along with human isolates, one dog Dirofilaria isolate was also subjected to PCR and sequencing of pan filarial primers cytochrome oxidase subunits 1 and 12S rDNA. We documented 78 cases of human dirofilariosis. The orbit, eyelid, and conjunctiva were the most commonly affected sites. Molecular characterization identified one dog and five human isolates as Candidatus Dirofilaria Hongkongensis. A rare case of subconjunctival infestation by B. malayi was also documented. Human dirofilariosis is a public health problem in the state of Kerala in India, and it is mostly caused by Candidatus Dirofilaria Hongkongensis. We propose that all diroifilaria isolates are subjected to sequencing for identification.
我们试图确定在喀拉拉邦的两家三级保健医院观察到的人类心丝虫病的流行病学和物种。我们从 2005 年 1 月至 2020 年 3 月搜索了医院数据库，以确定心丝虫病病例。除了人类分离株外，我们还对 1 只狗的心丝虫分离株进行了 PCR 和泛丝虫引物细胞色素氧化酶亚基 1 和 12S rDNA 的测序。我们记录了 78 例人类心丝虫病。眼眶、眼睑和结膜是最常受影响的部位。分子特征鉴定出一只狗和五个人类分离株为香港候选心丝虫。还记录了一例罕见的结膜下曼氏线虫感染病例。人类心丝虫病是印度喀拉拉邦的一个公共卫生问题，主要由香港候选心丝虫引起。我们建议对所有的心丝虫分离株进行测序鉴定。
