Endophytic Fungal Metabolite Research Laboratory, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode District, Tamil Nadu, India.
Endophytic Fungal Metabolite Research Laboratory, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode District, Tamil Nadu, India.
Environ Res. 2021 Jun;197:111147. doi: 10.1016/j.envres.2021.111147. Epub 2021 Apr 17.
The study aims at the Isolation, screening and antibacterial evaluation of Camptothecin (CPT) and its derivatives, an anticancer molecule from endophytic fungus Diaporthe caatingaensis MT192326 of the medicinal plant, Buchanania axillaris. Plant parts were collected from Sathyamangalam Tiger Reserve forest, Tamil Nadu. The fungus was isolated using DEKM07 medium was used as the screening medium for the presence of CPT. The strain with the highest yield of CPT was identified at the molecular level by 18S rDNA sequencing. CPT was isolated and analyzed by UV-Vis spectrophotometry, Thin layer chromatography, High-Performance Liquid Chromatography, Fourier Transform Infrared spectroscopy, and Electron spray ionization-mass spectrometry. The compounds identified by ESI-MS from the fungal extract were studied for their antibacterial assays against procured MTCC bacterial pathogens. The maximum yield of 0.681 mg/L of CPT was produced by the fungus D.caatingaensis. CPT derivatives were identified at m/z of 305, 348 and 389 through ESI-MS analysis. Antibacterial studies revealed that the endophytic fungal extract compounds were studied for antibacterial activities of disc diffusion assay, exhibiting a growth inhibition range of 15-22 mm in nutrient agar plate medium. The Minimum Inhibitory Concentration revealed the antibacterial potential at a lower concentration of 12.5-25 μg/ml with all bacteria studied. The relatively lower antimicrobial efficacy of partially purified bio-metabolites than the positive control streptomycin (3.125) concentration could be due to the presence of derivatives of the compounds that hinder the activity of the biometabolite. This is the first initiative to screen, isolate and analyze the antibacterial assays of CPT and derivatives from endophytic fungus D.caatingaensis of ethnopharmacologically important B.axillaris plant from STRF.
本研究旨在从药用植物黄桐内生真菌 Diaporthe caatingaensis MT192326 中分离、筛选和评价喜树碱（CPT）及其衍生物，这是一种抗癌分子。植物部分来自泰米尔纳德邦的 Sathyamangalam 老虎保护区森林。使用 DEKM07 培养基从植物部分中分离真菌，该培养基被用作存在 CPT 的筛选培养基。通过 18S rDNA 测序，在分子水平上鉴定出具有最高 CPT 产量的菌株。通过紫外可见分光光度法、薄层层析、高效液相色谱、傅里叶变换红外光谱和电子喷雾电离质谱对 CPT 进行分离和分析。通过 ESI-MS 从真菌提取物中鉴定出的化合物，研究了它们对采购的 MTCC 细菌病原体的抗菌活性。真菌 D.caatingaensis 产生的 CPT 最大产量为 0.681 mg/L。通过 ESI-MS 分析鉴定出 CPT 衍生物的质荷比为 305、348 和 389。通过抑菌圈扩散法研究了内生真菌提取物化合物的抗菌活性，在营养琼脂平板培养基中显示出 15-22mm 的生长抑制范围。最低抑菌浓度显示，所有研究的细菌在较低浓度 12.5-25μg/ml 下均具有抗菌潜力。与阳性对照链霉素（3.125）浓度相比，部分纯化生物代谢物的抗菌效果相对较低，可能是由于存在化合物的衍生物，这些衍生物阻碍了生物代谢物的活性。这是首次从 STRF 中具有民族药理学重要性的黄桐内生真菌 D.caatingaensis 中筛选、分离和分析 CPT 及其衍生物的抗菌活性。
