Beijing Key Laboratory of Bioelectromagnetism, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing, China.
Key Laboratory for Nano-Bio Interface Research, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, China.
J Neurosci Res. 2021 Jul;99(7):1850-1863. doi: 10.1002/jnr.24839. Epub 2021 Apr 12.
The application of electric field stimulation (EFS) can reduce the cation influx after spinal cord injury. However, regenerated cation influx and reestablished injury potential are observed after EFS. Polyethylene glycol (PEG) is popular as an effective cell membrane fusion agent. This study aims to determine the effects of the combination therapy of EFS and PEG in the ex vivo spinal cord after compression. The ex vivo spinal cords of female rats with compression injury were incubated in a double sucrose gap recording chamber (DSGRC) and randomly divided into the following four groups: (1) compression group: compression only, (2) EFS group: EFS for 15 min, (3) PEG group: PEG treatment for 4 min, and (4) EFS + PEG group: EFS for 15 min and PEG treatment for 4 min. The hematoxylin-eosin staining was performed to measure the necrotic area of the spinal cords. The gap potential was detected, and the area under the curve of the gap potential was calculated. The intracellular cation concentration, membrane permeability, and compound action potential were measured and quantified. Results revealed no significant difference in the necrotic areas among different groups, and the compression model of the ex vivo spinal cord in the DSGRC had high consistency and stability. The combination therapy could attenuate cation inflow, promote cell membrane restoration, and promote the functional recovery of the spinal cord conduction after compression in ex vivo spinal cords.
电场刺激（EFS）的应用可以减少脊髓损伤后的阳离子内流。然而，在 EFS 后会观察到再生阳离子内流和重新建立的损伤电位。聚乙二醇（PEG）作为一种有效的细胞膜融合剂而广受欢迎。本研究旨在确定 EFS 和 PEG 联合治疗在体外压缩后脊髓中的作用。雌性大鼠的体外压缩损伤脊髓在双蔗糖间隙记录室（DSGRC）中孵育，并随机分为以下四组：（1）压缩组：仅压缩，（2）EFS 组：EFS 治疗 15 分钟，（3）PEG 组：PEG 处理 4 分钟，（4）EFS+PEG 组：EFS 治疗 15 分钟和 PEG 处理 4 分钟。进行苏木精-伊红染色以测量脊髓的坏死区域。检测间隙电位，并计算间隙电位的曲线下面积。测量和量化细胞内阳离子浓度、膜通透性和复合动作电位。结果表明，不同组之间的坏死区域没有显著差异，并且 DSGRC 中的体外脊髓压缩模型具有高度的一致性和稳定性。联合治疗可以减轻阳离子内流，促进细胞膜修复，并促进体外脊髓压缩后脊髓传导的功能恢复。
