Nanchong Key Laboratory of Disease Prevention, Control, and Detection in Livestock and Poultry, Nanchong Vocational and Technical College, Nanchong 637131, China.
Pol J Vet Sci. 2021 Mar;24(1):135-143. doi: 10.24425/pjvs.2021.136802.
Alveolar macrophages (AMs) are not only important immune cell of the host, but also important target cell of a variety of respiratory pathogens. They play an important role in defense against pathogen invasion and in maintaining tissue balance. Interferon (IFN)-γ is a well known multipotent cytokine that has antiviral and antibacterial immune activity and enhances antigen presentation. To better reveal the biological processes of porcine AMs activated by IFN-γ, transcriptomic analysis was performed using Illumina HiSeqTM technique. The results identified 2,248 differentially expressed genes (DEGs), of which 753 were upregulated and 1,495 were downregulated, in porcine AMs 12 h after IFN-γ stimulation, compared with mock-treated porcine AMs. A gene ontology function enrichment analysis of these DEGs indicated that these genes were significantly enriched in functional clusters such as immune response, defense response, and intracellular signaling cascades. Analyzing the Kyoto Encyclopedia of Genes and Genomes pathways of the DEGs showed that these genes are mainly involved in cytokine-cytokine receptor interactions, alpha linolenic acid metabolism, and the RIG-I-like receptor signaling pathway. This study shows that a massive gene expression change occurred in porcine AMs following IFN-γ stimulation, which is critical for understanding the mechanisms of IFN-γ-mediated macrophage activation and immune regulation.
肺泡巨噬细胞（AMs）不仅是宿主重要的免疫细胞，也是多种呼吸道病原体的重要靶细胞。它们在抵御病原体入侵和维持组织平衡方面发挥着重要作用。干扰素（IFN）-γ是一种众所周知的多功能细胞因子，具有抗病毒和抗菌免疫活性，并增强抗原呈递。为了更好地揭示 IFN-γ激活的猪 AMs 的生物学过程，我们使用 Illumina HiSeqTM 技术进行了转录组分析。结果鉴定出 IFN-γ刺激后 12 小时猪 AMs 中有 2248 个差异表达基因（DEGs），其中 753 个上调，1495 个下调。这些 DEGs 的基因本体功能富集分析表明，这些基因显著富集在免疫反应、防御反应和细胞内信号级联等功能簇中。对 DEGs 的京都基因与基因组百科全书通路分析表明，这些基因主要参与细胞因子-细胞因子受体相互作用、α-亚麻酸代谢和 RIG-I 样受体信号通路。本研究表明，IFN-γ刺激后猪 AMs 发生了大量基因表达变化，这对于理解 IFN-γ 介导的巨噬细胞激活和免疫调节机制至关重要。
