Chemistry Department, Lomonosov Moscow State University, Moscow, Russian Federation.
Engelhardt Institute of Molecular Biology RAS, Moscow, Russian Federation.
Nucleosides Nucleotides Nucleic Acids. 2021;40(5):559-577. doi: 10.1080/15257770.2021.1910297. Epub 2021 Apr 13.
Aptamers are structured oligonucleotides that specifically bind their targets. Oligonucleotides can be assembled in large nanostructures via intermolecular duplexes or G-quadruplexes. Addition of aptamers can be used to create nanostructures that bind specifically certain targets. Here two types of self-assembling locks were used to create bimodular aptamer constructions. Well-known aptamer to thrombin was chosen as a model object. The assembly of duplex locks was more efficient at low concentrations. The functional activity of aptamer modules was nearly the same as in HD1. However, the affinity of bimodular aptamers with G-quadruplex locks to immobilized thrombin was 5-10 times higher.
适体是特异性结合其靶标的结构寡核苷酸。寡核苷酸可以通过分子间的双链体或 G-四联体组装成大型纳米结构。添加适体可用于创建特异性结合特定靶标的纳米结构。在这里，两种类型的自组装锁被用于创建双模适体结构。选择著名的凝血酶适体作为模型对象。在低浓度下，双链体锁的组装效率更高。适体模块的功能活性与 HD1 几乎相同。然而，具有 G-四联体锁的双模适体与固定化凝血酶的亲和力高 5-10 倍。
