Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Center for Biosafety Research and Strategy, Tianjin University, Tianjin, 300072, China.
Environ Microbiol. 2021 May;23(5):2692-2703. doi: 10.1111/1462-2920.15515. Epub 2021 Apr 19.
Labelling of Type IV pili (TFP) can greatly improve our understanding of the pivotal roles of TFP in a variety of bacterial activities including motility, surface sensing and DNA-uptake etc. Here we show a simple and switchable pili-labelling method by plasmid-based inducible replacement of PilA without genetic modification in bacterial genome employed by complicated methods. Using this method, we characterized pili morphology and twitching motility of Pseudomonas aeruginosa in details. More importantly, we demonstrate its application in studying the replenishment dynamics of pilin pool of P. aeruginosa.
通过质粒诱导替换 PilA 而不改变细菌基因组的复杂方法，我们展示了一种简单且可切换的菌毛标记方法。利用这种方法，我们详细研究了铜绿假单胞菌的菌毛形态和扭动运动。更重要的是，我们证明了它在研究铜绿假单胞菌菌毛库补充动力学中的应用。
