Gastrointestinal secretions and inflammation research unit, Department of Physiology, University of Ibadan, Ibadan, Oyo State, Nigeria.
Can J Physiol Pharmacol. 2021 Oct;99(10):989-999. doi: 10.1139/cjpp-2020-0678. Epub 2021 Apr 13.
Potassium bromate (KBrO) present in consumed ozonised water was recently documented to exacerbate experimental gastric ulcer. Information, however, is vague as regards its effects in the colon where water reabsorption occurs. In this study, we observed the possible effects of KBrO on oxidative stress and inflammatory biomarkers in sodium hydroxide (NaOH) - induced Crohn's colitis (CC). Wistar rats (180-200 g) were divided into six groups ( = 10): () control; () untreated CC (induced by 1.4% NaOH; intra-rectal administration); and (-) CC treated with vitamin E, KBrO, vitamin E+KBrO, and sulphazalazine, respectively, for 7 days. Body weight and stool score were monitored daily. By day 3 and 7, excised colon was evaluated for ulcer scores and biochemical and histological analysis. Blood samples collected on days 3 and 7 were assayed for haematological indices using standard methods. Data were subjected to analysis of variance (ANOVA) and ≤ 0.05 considered significant. Platelet/lymphocyte ratio, colonic ulcer score, malondialdehyde, and mast cells were significantly decreased while colonic sulfhydryl, and Ca- and Na/K-ATPase activities were increased following KBrO treatment compared with untreated CC. These findings suggest that KBrO may mitigate against NaOH-induced CC via inhibiting mast cell population and oxidative and inflammatory content but stimulating colonic sulfhydryl and Ca- and Na/K-ATPase activities.
臭氧氧化后的水中存在的溴酸钾（KBrO）最近被记录会加剧实验性胃溃疡。然而，关于其在水吸收发生的结肠中的影响，信息尚不清楚。在这项研究中，我们观察了 KBrO 对氢氧化钠（NaOH）诱导的克罗恩病（CC）中氧化应激和炎症生物标志物的可能影响。将 180-200 克的 Wistar 大鼠分为六组（每组 10 只）：（）对照组；（）未治疗的 CC（通过 1.4%NaOH 经直肠给药诱导）；和（-）CC 分别用维生素 E、KBrO、维生素 E+KBrO 和磺胺嘧啶治疗 7 天。每天监测体重和粪便评分。在第 3 天和第 7 天，切除结肠进行溃疡评分和生化及组织学分析。在第 3 天和第 7 天收集的血液样本采用标准方法检测血液学指标。数据采用方差分析（ANOVA）进行分析，≤0.05 被认为具有统计学意义。与未经治疗的 CC 相比，KBrO 治疗后血小板/淋巴细胞比值、结肠溃疡评分、丙二醛和肥大细胞显著降低，而结肠巯基、Ca 和 Na/K-ATP 酶活性增加。这些发现表明，KBrO 可能通过抑制肥大细胞群以及氧化和炎症物质，同时刺激结肠巯基和 Ca 和 Na/K-ATP 酶活性，减轻 NaOH 诱导的 CC。
