Agatonovic-Kustrin Snezana, Ramenskaya Galina, Kustrin Ella, Morton David W
A.P. Arzamastsev Department of Pharmaceutical and Toxicological Chemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russian Federation; School of Pharmacy and Biomedical Sciences, La Trobe University, Edwards Rd, Bendigo 3550, Australia.
A.P. Arzamastsev Department of Pharmaceutical and Toxicological Chemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russian Federation; Scientific Center for Expert Evaluation of Medicinal Products, Ministry of Health of the Russian Federation, 8/2 Petrovskii Blvd., Moscow 127051, Russian Federation.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Mar 29;1173:122676. doi: 10.1016/j.jchromb.2021.122676.
A high-performance thin-layer chromatography with microchemical derivatization and bioassay guided detection was used for bioanalytical profiling of selected marigold plant extracts. Anisaldehyde/sulfuric acid reagent and thymol/sulfuric acid reagent were used to visualize separated components on the chromatograms. Antioxidant activity and α-amylase inhibition were assessed with 2 bioassays, DPPH assay to detect free radical scavengers and starch-iodineassay method to detect compounds that inhibit α-amylase. The highest antioxidant activity of 10.12 μg of gallic acid equivalents (GAE) per 20 µL of extract was measured in extract from Tagetes flowers and the lowest in the extract from Calendula leaves with 5.10 μg of GAE. Multiple zones of α-amylase inhibition were detected. A detailed analysis of the ATR-FTIR spectra from the bands at R = 0.24 suggest that faradiol esters and saturated fatty acids esters, palmitic acid, myristic acid, and lauric acid are responsible for α-amylase inhibition, unsaturated fatty acids for the band at R = 0.51 and phytoecdysteroids for the band at R = 0.53.
采用具有微化学衍生化和生物测定引导检测功能的高效薄层色谱法，对选定的万寿菊植物提取物进行生物分析表征。使用茴香醛/硫酸试剂和百里酚/硫酸试剂使色谱图上分离的成分可视化。通过两种生物测定法评估抗氧化活性和α-淀粉酶抑制作用，即采用DPPH测定法检测自由基清除剂，采用淀粉-碘测定法检测抑制α-淀粉酶的化合物。在万寿菊花朵提取物中测得的最高抗氧化活性为每20微升提取物含10.12微克没食子酸当量（GAE），而在金盏花叶提取物中最低，为5.10微克GAE。检测到多个α-淀粉酶抑制区。对R = 0.24处谱带的衰减全反射傅里叶变换红外光谱（ATR-FTIR）进行详细分析表明，法呢二醇酯和饱和脂肪酸酯、棕榈酸、肉豆蔻酸和月桂酸是α-淀粉酶抑制作用的原因，不饱和脂肪酸导致R = 0.51处的谱带，而植物蜕皮甾酮导致R = 0.53处的谱带。
